FIGURE

Fig. S1

ID

ZDB-FIG-111223-16

Publication

Blum et al., 2012 - Retinoic acid signaling controls the formation, proliferation and survival of the blastema during adult zebrafish fin regeneration

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Fig. S1

The hsp70:cyp26a1 line efficiently inhibits RA signaling. (A) Heat shock of hsp70:cyp26a1 embryos for 1 hour starting at 6 hpf results in close proximity of the first somite to the otic vesicle and a kink at the head-trunk boundary at 24 hpf, representative of complete loss of RA signaling. (B) Whole-mount in situ hybridization for egr2a, a marker of hindbrain rhombomeres 3 and 5, illustrates absence of rhombomere 5 in hsp70:cyp26a1 embryos, caused by posterior expansion of more anterior rhombomeres. hs, heat shock; o, otic vesicle; s, first somite; 3, rhombomere 3; 5, rhombomere 5. Scale bars: 100 μm.

Expression Data

Gene:	egr2a
Fish:	WT kn1Tg/+
Condition:	heat shock
Anatomical Terms:	rhombomere 3 rhombomere 5
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kn1Tg/+
Condition:	heat shock
Observed In:	head otic vesicle
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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