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ZIRC
ZFIN ID: ZDB-FIG-101123-17
Murphy et al., 2010 - Disruption of angiogenesis and tumor growth with an orally active drug that stabilizes the inactive state of PDGFR{beta}/B-RAF. Proceedings of the National Academy of Sciences of the United States of America   107(9):4299-4304 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Long-pec

Fig. S3 Labeling of the Tg:fli1-egfp zebrafish vasculature and quantification of the drug treatment effect on the intersegmental vessel volume. (A) Images are taken from Fig. 3A with major vascular structures labeled. In the head: MCeV, middle cerebral vein; MsV, mesencephalic vein; PHS, primary head sinus; IOC, inner optic circle; PrA, prosencephalic artery. In the tail: DLAV, dorsal longitudinal anastomotic vessel; DA, dorsal aorta; PCV, posterior cardinal vein; ISV, intersegmental vessel; VTA, vertebral artery. (B) Representative views of zebrafish embryos treated as in Fig. 3A (images represent merged phase and GFP fluorescence views of the head and trunk regions of Tg:fli1-EGFP embryos). (Scale bar, 200 μm.) (C) Quantification of ISV volume from embryos treated as in Fig. 3A. (D) Quantification of ISV volume from embryos treated as in Fig. 3B. To measure the ISV volume, individual ISVs were digitally isolated using the Imaris countersurface/isosurface functions. Thirty independent ISVs from four independent embryos were used for the measurement. Reported ± SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP y1Tg chemical treatment: pharmaceutical Long-pec vasculature IFL
y1Tg chemical treatment: semaxanib Long-pec vasculature IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA