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ZIRC
ZFIN ID: ZDB-FIG-101021-33
Lyons et al., 2010 - Carboxypeptidase A6 in zebrafish development and implications for VIth cranial nerve pathfinding. PLoS One   5(9):e12967 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 5

Fig. 3 Temporal and spatial expression of zebrafish CPA genes.

(A, B) qPCR was performed using cDNA prepared from zebrafish at the indicated developmental time points. All values were normalized to β-actin, and are shown in (A) as expression relative to CPA1 mRNA at 2 dpf, or in (B) as expression of each gene relative to its highest level (100%). (C, D) In situ hybridization was used to determine the spatial distribution of CPA mRNA expression. (C) At 2 dpf CPA5 mRNA (arrows) was detected in a mast cell population and CPA6 mRNA was seen in precursor tissues found in the stomodeum, posterior to the eyes, and in the pectoral fin buds. (D) At 4 dpf, mRNA for CPA1, 2, 4, and 5 were expressed in the pancreas.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cpa1 WT standard conditions Day 4 pancreas ISH
Day 5 whole organism RTPCR
cpa2 WT standard conditions Day 4 pancreas ISH
Day 5 whole organism RTPCR
cpa4 WT standard conditions Prim-5 to Day 5 whole organism RTPCR
Day 4 pancreas ISH
cpa5 WT standard conditions Long-pec myeloid cell ISH
Day 4 pancreas ISH
Day 5 whole organism RTPCR
cpa6 WT standard conditions Prim-5 to Day 5 whole organism RTPCR
Long-pec head ISH
Long-pec pectoral fin bud ISH
Long-pec stomodeum ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One