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ZFIN ID: ZDB-FIG-100830-7
Hsu et al., 2010 - Multiple domains in the Crumbs Homolog 2a (Crb2a) protein are required for regulating rod photoreceptor size. BMC Cell Biology   11:60 Full text @ BMC Cell Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Day 6

Fig. 5 The zs-4 antibody recognizes the extracellular domain of Crb2a. (A-A″) Single confocal z-section of the photoreceptor layer in a 6 d wild-type retina labeled with rabbit panCrb antibodies (A) and zs-4 monoclonal (A′) and the two channels merged (A″). The arrow indicates where anti-panCrb and zs-4 labeling does not appear to fully overlap. (B-C″) Confocal z-projection of a 6 d retina labeled with anti-HA antibody (monoclonal IgG3; red) and zs-4 antibody (blue) and merged with anti-GFP (green). (B-B″) Wild-type retina. (C-C″) Crb2aExtra_TM transgenic retina. Endogenous Crb2a labeling is weak in comparison to Crb2aExtra_TM protein (C″). (D-F′) Confocal z-projection of the photoreceptor layer in a 6 d retina labeled with zs-4 antibody (red), rods express GFP (green). TO-PRO-3 (blue) labeling reveals the position of nuclei in the photoreceptor layer. (D-D′) Wild-type retina. (E-E″) Crb2aExtra_Secr transgenic retina. Single z-section from the white boxed area in E′, showing zs-4 labeling around the inner segment (indicated by yellow bracket and outlined in yellow) of a double-cone, the cell-body is circled in white (E″). (F-F′) Crb2aExtra_Secr transgenic retina where no rods are present. (G-H) Single z-section of the brain ventricular region of a 40 hour postfertilization embryo labeled with zs-4 antibody (red) showing ventricular surface labeling. Cell nuclei are labeled with TO-PRO-3 (blue). (G) Wild-type embryo. (H) ome (crb2a) mutant embryo. The midline (where the ventricles would normally form) is indicated by the white arrow, and eyes indicated by dashed white lines. Scale bars, 5 μm (A-A″′, D-F′) 10 μm (G, H).

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab2-crb IHC slc45a2b4/b4; fl1Tg standard conditions Day 6 retinal photoreceptor layer
IHC slc45a2b4/b4; fl1Tg; umz4Tg standard conditions Day 6 retinal photoreceptor layer
IHC slc45a2b4/b4; fl1Tg; umz7Tg standard conditions Day 6 retinal photoreceptor layer
zs-4 IHC WT standard conditions Prim-25 ventricular system
IHC crb2am98/m98 standard conditions Prim-25 Not Detected ventricular system
IHC slc45a2b4/b4; fl1Tg standard conditions Day 6 retinal photoreceptor layer
IHC slc45a2b4/b4; fl1Tg; umz4Tg standard conditions Day 6 retinal photoreceptor layer
IHC slc45a2b4/b4; fl1Tg; umz7Tg standard conditions Day 6 retinal photoreceptor layer
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Cell Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.
Open Access.
Full text @ BMC Cell Biol.