ZFIN ID: ZDB-FIG-100806-52
Bussmann et al., 2010 - Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk. Development (Cambridge, England)   137(16):2653-2657 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5

Fig. 1 Generation and visualization of flt1enh:RFP and SAGFF27C;UAS:GFP transgenic lines. (A-C) Arterial-restricted reporter expression in the flt1enh:RFP transgenic line at 5 dpf (A), compared with kdrl:GFP, which labels all blood endothelial cells (B) allows for discrimination between ISVs connected to the dorsal aorta or PCV. (C) Overlay of A,B. (D) Schematic representation of flt1enh:RFP expression. (E) Lymphatic reporter expression in the SAGFF27C;UAS:GFP transgenic line at 5 dpf, compared with an angiography (F) that labels perfused blood vessels (red in G). (G) Overlay of E,F. (H) Schematic representation of SAGFF27C;UAS:GFP expression. aISV, arterial ISV; DA, dorsal aorta; DLLV, dorsal longitudinal lymphatic vessel; ISLV, intersegmental lymphatic vessel; PCV, posterior cardinal vein; TD, thoracic duct; vISV, venous ISV.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP hu5333Tg; s843Tg standard conditions Day 5 artery intersegmental vessel IFL
Day 5 dorsal aorta IFL
Day 5 intersegmental vein blood vessel endothelial cell IFL
Day 5 posterior cardinal vein blood vessel endothelial cell IFL
map3k20nksagff27cGt/+; nkuasgfp1aTg standard conditions Day 5 dorsal longitudinal lymphatic vessel IFL
Day 5 intersegmental lymph vessel lymph vessel endothelium IFL
Day 5 thoracic duct IFL
RFP hu5333Tg; s843Tg standard conditions Day 5 artery intersegmental vessel IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development