ZFIN ID: ZDB-FIG-100806-52
Bussmann et al., 2010 - Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk. Development (Cambridge, England)   137(16):2653-2657 Full text @ Development
Anatomical Terms:
Stage: Day 5

Fig. 1 Generation and visualization of flt1enh:RFP and SAGFF27C;UAS:GFP transgenic lines. (A-C) Arterial-restricted reporter expression in the flt1enh:RFP transgenic line at 5 dpf (A), compared with kdrl:GFP, which labels all blood endothelial cells (B) allows for discrimination between ISVs connected to the dorsal aorta or PCV. (C) Overlay of A,B. (D) Schematic representation of flt1enh:RFP expression. (E) Lymphatic reporter expression in the SAGFF27C;UAS:GFP transgenic line at 5 dpf, compared with an angiography (F) that labels perfused blood vessels (red in G). (G) Overlay of E,F. (H) Schematic representation of SAGFF27C;UAS:GFP expression. aISV, arterial ISV; DA, dorsal aorta; DLLV, dorsal longitudinal lymphatic vessel; ISLV, intersegmental lymphatic vessel; PCV, posterior cardinal vein; TD, thoracic duct; vISV, venous ISV.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP hu5333Tg; s843Tg standard conditions Day 5 artery intersegmental vessel IFL
Day 5 dorsal aorta IFL
Day 5 intersegmental vein blood vessel endothelial cell IFL
Day 5 posterior cardinal vein blood vessel endothelial cell IFL
map3k20nksagff27cGt/+; nkuasgfp1aTg standard conditions Day 5 dorsal longitudinal lymphatic vessel IFL
Day 5 intersegmental lymph vessel lymph vessel endothelium IFL
Day 5 thoracic duct IFL
RFP hu5333Tg; s843Tg standard conditions Day 5 artery intersegmental vessel IFL
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development