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ZFIN ID: ZDB-FIG-100806-55
Bussmann et al., 2010 - Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk. Development (Cambridge, England)   137(16):2653-2657 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 4 Intersegmental arteries are required for LEC migration. (A-C) flt1enh:RFP (A), SAGFF27C;UAS:GFP (B) and merged (C) unilateral confocal images of control embryos at 4.5 dpf showing lymphatic patterning along aISVs in normal blood vasculature. Arrows indicate ISLVs. (D-F) flt1enh:RFP (D), SAGFF27C;UAS:GFP (E) and merged (F) unilateral confocal images of kdrl morpholino injected embryos at 4.5 dpf showing LEC migration along arteries that cross-connect abnormally at the horizontal myoseptum, and that do not extend into the dorsal half (indicated by yellow asterisk) of the respective somite. Arrows indicate LECs along the surface of an abnormal aISV. Vertical red bars indicate the DA. (G) Model of lymphatic patterning within the zebrafish trunk. Secondary sprouts from the PCV that do not connect to primary intersegmental vessels migrate to the horizontal myoseptum region where they constitute a pool of PLs. PLs will migrate ventrally or dorsally to form the thoracic duct and ISLVs, respectively, and use arteries as their substrate.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP map3k20nksagff27cGt/+; hu5333Tg; nkuasgfp1aTg standard conditions Day 4 intersegmental lymph vessel lymph vessel endothelium IFL
Day 4 thoracic duct IFL
map3k20nksagff27cGt/+; hu5333Tg; nkuasgfp1aTg + MO1-kdrl standard conditions Day 4 intersegmental lymph vessel lymph vessel endothelium IFL
Day 4 thoracic duct IFL
RFP map3k20nksagff27cGt/+; hu5333Tg; nkuasgfp1aTg standard conditions Day 4 artery intersegmental vessel IFL
map3k20nksagff27cGt/+; hu5333Tg; nkuasgfp1aTg + MO1-kdrl standard conditions Day 4 artery intersegmental vessel IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
map3k20nksagff27cGt/+; hu5333Tg; nkuasgfp1aTg + MO1-kdrl standard conditions Day 4 intersegmental artery malformed, abnormal
Day 4 intersegmental artery mislocalised, abnormal
Day 4 intersegmental lymph vessel malformed, abnormal
Day 4 intersegmental lymph vessel mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development