FIGURE

Fig. 5

ID

ZDB-FIG-100802-39

Publication

Gao et al., 2010 - Oda16/Wdr69 is essential for axonemal dynein assembly and ciliary motility during zebrafish embryogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Wdr69 is needed for ciliary motility and outer arm dynein assembly. A,B: Tracking software was used to follow the flow of fluorescent beads injected into Kupffer′s vesicle (KV) of live embryos. Tracks are superimposed on images of KV. A: In control morphants, beads flowed in a circular counterclockwise direction. B: This directional flow was lost in wdr69 MO^AUG morphants, where beads bounced around randomly. C: Velocity of bead movement was significantly reduced in wdr69 knockdown embryos (n = 25 beads from 5 embryos) relative to controls (n = 28 beads from 6 embryos). Error bars=one standard deviation. D,E: Electron microscopy of KV cilia. D: In control MO injected embryos, typical outer dynein arms (arrow) were present on all nine outer doublets of all cilia examined. E: In contrast, KV cilia from wdr69 MO^AUG morphants displayed greatly reduced numbers of outer dynein arms. MO, morpholino oligonucleotides.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-daw1
Knockdown Reagent:	MO1-daw1
Observed In:	cilium movement Kupffer's vesicle axonemal dynein complex Kupffer's vesicle axoneme Kupffer's vesicle cilium
Stage Range:	5-9 somites to 10-13 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.