FIGURE

Fig. S1

ID

ZDB-FIG-100723-37

Publication

Freisinger et al., 2010 - Regulator of g protein signaling 3 modulates wnt5b calcium dynamics and somite patterning

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

rgs3 expression is adjacent and overlapping with wnt5b, related to Figure 1. Temporal and spatial expression of rgs3 compared to wnt5b in zebrafish development. Whole Mount In Situ Hybridization was utilized to compare the spatial expression of wnt5b to rgs3. WMISH of 14hpf (A–D) and 24hpf (E–F) Wt embryos. Lateral (A–C and E) and dorsal (D and F) views illustrate that rgs3 is expressed in the developing somites and posterior tail (A–E). Co localization of wnt5b and rgs3, determined by double label WMISH with wnt5b (red) and rgs3 (blue), shows adjacent and overlapping expression domains around Kupffer′s vesicle (C) and in the tailbud (D). Double label WMISH with rgs3 (blue) and engrailed1 (red) highlight that rgs3 is expressed in the midbrain/hindbrain boundary (E). Sense probes (negative control) gave no specific hybridization signal.

Expression Data

Genes:	rgs3a wnt5b
Fish:	WT
Anatomical Terms:	Kupffer's vesicle midbrain hindbrain boundary somite tail bud posterior region
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.