Fig. 7
- ID
- ZDB-FIG-100719-26
- Publication
- Say et al., 2010 - A Functional Requirement for PAK1 Binding to the KH(2) Domain of the Fragile X Protein-Related FXR1
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The PAK1 Interaction Is Required for the Physiological Function of FXR1 (A) FXR1(Q348K/E352A) cannot rescue developmental defects in muscle development in zebrafish. Assays were performed as detailed in Figure 4. Synthetic mRNA encoding FXR1(I304N) or FXR1(Q348K/E352A) could not rescue the somite defects associated with loss of FXR1. The human FMR1, although able to bind PAK1, was unable to rescue FXR1 loss, indicating different requirements in terms of muscle development. (B) Confocal images of embryos treated with FXR1 MO (1.5 ng per embryo) or in combination with the PAK1 inhibitor IPA3 (10 μM) added at 7 hpf (i.e., after gastrulation). Representative images show organization of myosin heavy chain (at 24 hpf); IPA3 treatment enhances the severity of the effects of FXR1 loss. (C) Rescue of FXR1 MO-mediated knockdown using a triple mutant (TM) PAK1 binding-deficient FXR1(Q348K/E352A/S420D) can allow a gain of function, unlike the FXR1(Q348K/E352A) = KA. The panels show typical phenotypes of three morphant and three FXR1-TM rescue embryos at 24 hpf. Note the clear somite boundaries observed in the case of rescued embryos. In these experiments, quantification of the phenotypes in embryos was carried out after embryos were selected at 6 hpf for equal injection of MO and mRNA co-injected with Texas-red dextran. The table indicates the percentage of embryos in each category scored as for (B), with n being the number of embryos scored for each injection. |
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| Stage: | Prim-5 |