Fig. 2

Specimen in a Corrected Optical Rotational Enclosure (SCORE) imaging of living animals. Zebrafish imaging is facilitated by use of a fluoro-carbon tube for housing. (A) Zebrafish images taken in polymer tubing produce a distorted image (arrows) due to the change in refractive index between the tubing and the air. Cartoon images representing a fish immobilized using traditional techniques on a skewed plane (B), a larval fish within a polymer tube without a corrective solution producing distortion (C), and within a corrective solution to produce a clear image (D). Fluorinated ethylene propylene (FEP) tubing produces a clear image at 50x (E ) (also see Supplemental Movie S1, available online at www.liebertonline.com), 100x (F), and 200x (G) of a 5 day postfertilization (dpf) larvae. A 5 dpf Tg(gata-1:DsRed)sd2 larvae in FEP tubing produces a clear background-free image at 50x (H) (also see Supplemental Movie S2, available online at www.liebertonline.com) and 100x (I). A 5 dpf Tg(fli:eGFP)y1 in FEP tubing produces a clear background-free image at 50x (J) (also see Supplemental Movie S3, available online at www.liebertonline.com) and 100x (K).