PUBLICATION
SCORE imaging: specimen in a corrected optical rotational enclosure
- Authors
- Petzold, A.M., Bedell, V.M., Boczek, N.J., Essner, J.J., Balciunas, D., Clark, K.J., and Ekker, S.C.
- ID
- ZDB-PUB-100614-20
- Date
- 2010
- Source
- Zebrafish 7(2): 149-154 (Journal)
- Registered Authors
- Balciunas, Darius, Clark, Karl, Ekker, Stephen C., Essner, Jeffrey, Petzold, Andrew
- Keywords
- none
- MeSH Terms
-
- Anatomy/methods*
- Animals
- Diagnostic Imaging/methods*
- Glycerol
- Polytetrafluoroethylene/analogs & derivatives
- Specimen Handling/methods*
- Zebrafish/anatomy & histology*
- PubMed
- 20528262 Full text @ Zebrafish
Citation
Petzold, A.M., Bedell, V.M., Boczek, N.J., Essner, J.J., Balciunas, D., Clark, K.J., and Ekker, S.C. (2010) SCORE imaging: specimen in a corrected optical rotational enclosure. Zebrafish. 7(2):149-154.
Abstract
Visual data collection is paramount for the majority of scientific research. The added transparency of the zebrafish (Danio rerio) allows for a greater detail of complex biological research that accompanies seemingly simple observational tools. We developed a visual data analysis and collection approach that takes advantage of the cylindrical nature of the zebrafish allowing for an efficient and effective method for image capture that we call Specimen in a Corrected Optical Rotational Enclosure imaging. To achieve a nondistorted image, zebrafish were placed in a fluorinated ethylene propylene tube with a surrounding optically corrected imaging solution (water). By similarly matching the refractive index of the housing (fluorinated ethylene propylene tubing) to that of the inner liquid and outer liquid (water), distortion was markedly reduced, producing a crisp imagable specimen that is able to be fully rotated 360 degrees. A similar procedure was established for fixed zebrafish embryos using convenient, readily available borosilicate capillaries surrounded by 75% glycerol. The method described here could be applied to chemical genetic screening and other related high-throughput methods within the fish community and among other scientific fields.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping