ZFIN ID: ZDB-FIG-100520-24
Eames et al., 2010 - UDP xylose synthase 1 is required for morphogenesis and histogenesis of the craniofacial skeleton. Developmental Biology   341(2):400-415 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 6 Proteoglycan detection in wild-type and uxs1 mutant skeletons. (A–F) Whole-mount wheat germ agglutinin (WGA) staining to visualize N-acetylglucosamine, ventral views. (G–J) Whole-mount immunostaining against heparan sulfate (G, H) and chondroitin sulfate (I, J) proteoglycans, ventral views. Dissected pharyngeal cartilages revealed reduced WGA staining in uxs1hi3357 mutants (B, D), compared to wild-type siblings (A, C) at 5 dpf. Higher magnification of ceratohyal regions also showed that WGA-positive material was not deposited normally in mutants (D), compared to organized deposition in wild types (C). Dissected pectoral fins showed that both endoskeletal disc and actinotrichia had less WGA staining and fewer actinotrichia in uxs1hi3357 mutants (F), compared to wild-type siblings (E) at 5 dpf. Immunodetection of heparan sulfate demonstrated that HSPGs were localized to pharyngeal domains in wild type (G), but HSPGs were not detectable in homozygous uxs1hi3357 animals (H). Similarly, immunodetection of chondroitin sulfate was abundant in wild-type cartilages (I), but was absent in uxs1 mutants (J). Abbreviations: at, actinotrichia; cb1-5, ceratobranchials 1-5; ch, ceratohyal; ed, endoskeletal disc; m, Meckel′s cartilage; pq, palatoquadrate.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-10E4 IHC WT standard conditions Protruding-mouth ceratobranchial cartilage
IHC Protruding-mouth ceratohyal cartilage
IHC Protruding-mouth Meckel's cartilage
IHC Protruding-mouth palatoquadrate cartilage
IHC Protruding-mouth pectoral fin cartilage
IHC Protruding-mouth pharyngeal arch cartilage
IHC uxs1hi3357Tg/hi3357Tg standard conditions Protruding-mouth Not Detected ceratobranchial cartilage
IHC Protruding-mouth Not Detected ceratohyal cartilage
IHC Protruding-mouth Not Detected Meckel's cartilage
IHC Protruding-mouth Not Detected palatoquadrate cartilage
IHC Protruding-mouth Not Detected pectoral fin cartilage
IHC Protruding-mouth Not Detected pharyngeal arch cartilage
Ab-CS-56 IHC WT standard conditions Protruding-mouth ceratohyal cartilage
IHC Protruding-mouth Meckel's cartilage
IHC Protruding-mouth palatoquadrate cartilage
IHC uxs1hi3357Tg/hi3357Tg standard conditions Protruding-mouth Not Detected ceratohyal cartilage
IHC Protruding-mouth Not Detected Meckel's cartilage
IHC Protruding-mouth Not Detected palatoquadrate cartilage
Phenotype Details
Fish Conditions Stage Phenotype
uxs1hi3357Tg/hi3357Tg standard conditions Protruding-mouth chondroitin sulfate biosynthetic process disrupted, abnormal
Protruding-mouth heparan sulfate proteoglycan biosynthetic process disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 341(2), Eames, B.F., Singer, A., Smith, G.A., Wood, Z.A., Yan, Y.L., He, X., Polizzi, S.J., Catchen, J.M., Rodriguez-Mari, A., Linbo, T., Raible, D.W., and Postlethwait, J.H., UDP xylose synthase 1 is required for morphogenesis and histogenesis of the craniofacial skeleton, 400-415, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.