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ZFIN ID: ZDB-FIG-091215-66
Lee et al., 2009 - Notch Signaling Functions as a Cell-Fate Switch between the Endothelial and Hematopoietic Lineages. Current biology : CB   19(19):1616-1622 Full text @ Curr. Biol.
Anatomical Terms:
Stage: 14-19 somites

Fig. 3 Reduced Notch Activity Causes an Increase of Endothelial Cells at the Expense of Hematopoietic Cells

(A) Transverse sections of 18 hpf DMSO- or DAPT-treated embryos, visualized for TOPRO (white), kdrl:mCherry (red), and gata1:GFP (green). DAPT-treated embryos exhibited a concomitant gain of endothelial cells and loss of hematopoietic cells.

(B) Transverse sections of 20 hpf DMSO- or DAPT-treated phenotypic bloodless (bds) mutant embryos, visualized for TOPRO (white), kdrl:GFP (green), and phalloidin (red).

(C) Quantification of the number of endothelial nuclei per focal plane in DMSO-treated bds (n = 22), DAPT-treated bds (n = 20), and DAPT-treated wild-type sibling (n = 24) embryos. DAPT treatment in embryos lacking primitive hematopoietic cells (bds + DAPT) did not result in a change in the number of endothelial cells, suggesting that the ectopic endothelial cells observed in DAPT-treated wild-type embryos might have originated from cells that normally produce hematopoietic cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP la781Tg; s896Tg control 14-19 somites nucleate erythrocyte IFL
la781Tg; s896Tg chemical treatment: DAPT 14-19 somites nucleate erythrocyte IFL
mCherry la781Tg; s896Tg control 14-19 somites endothelial cell IFL
la781Tg; s896Tg chemical treatment: DAPT 14-19 somites endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.