ZFIN ID: ZDB-FIG-091016-26
Anderson et al., 2009 - Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration. Developmental Biology   334(1):213-223 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 6
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. 1 Initial formation and degeneration of the exocrine pancreas in dandelion (ddn) mutants. (A–C) Lateral views of WT (A), ddns872 (B), and ddns904 mutant (C) larvae at 100 hpf in the 2-color Liver, Insulin, acinar Pancreas (2CLIP) transgenic background (see Materials and methods). Acinar cells of the exocrine pancreas (xp) are green and hepatocytes (li) and pancreatic beta cells (b) are red. ddn mutants exhibit minimal acinar tissue, but overall larva morphology is only moderately affected. (D, E) Successive images of individual WT (D) and ddns872 mutant (E) larvae between 84 and 148 hpf in the 2CLIP; Tg(ptf1a:EGFP)jh1 background. The exocrine pancreas and liver grow larger in WT, but degenerate in ddn mutants. Hepatocyte fragments are found in the circulation (arrowheads). (F–K) 3-D projections of confocal stacks showing composition of WT (F, H, J) and ddns872 mutant (G, I, K) endocrine pancreas at 100 hpf in Tg(ins:dsRed)m1081 background. (F, G) A core of Tg(ins:dsRed)m1081+ cells (red) is surrounded by a mantle of Glucagon+ (Gcg) cells (green). Extrapancreatic duct (epd) structure is intact. (H, I) Isl-1, and (J, K) Somatostatin (Sst) positive cells appear unaffected. Arrowheads in G, I, and M point to endocrine cells outside of the primary islet. (L, M) Nkx6.1 immunostaining reveals the presence of intra-pancreatic duct cells in both WT and dnmt1s872 mutant larvae.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed dnmt1s872/s872; m1018Tg standard conditions Day 4 endocrine pancreas IFL
dnmt1s872/s872; m1018Tg; s854Tg standard conditions Day 4 endocrine pancreas pancreatic B cell IFL
m1018Tg; s854Tg standard conditions Day 4 endocrine pancreas pancreatic B cell IFL
EGFP dnmt1s872/s872; gz15Tg standard conditions Day 4 Not Detected exocrine pancreas IFL
dnmt1s872/s872; gz15Tg; jh1Tg standard conditions Protruding-mouth exocrine pancreas acinar cell IFL
Day 4 exocrine pancreas acinar cell IFL
Day 5 exocrine pancreas acinar cell IFL
Day 6 Not Detected exocrine pancreas IFL
dnmt1s904/s904; gz15Tg standard conditions Day 4 Not Detected exocrine pancreas IFL
gz15Tg standard conditions Day 4 exocrine pancreas acinar cell IFL
gz15Tg; jh1Tg standard conditions Protruding-mouth exocrine pancreas acinar cell IFL
Day 4 exocrine pancreas acinar cell IFL
Day 5 exocrine pancreas acinar cell IFL
Day 6 exocrine pancreas acinar cell IFL
GFP dnmt1s872/s872; m1018Tg; s854Tg standard conditions Day 4 gall bladder IFL
Day 4 pancreas IFL
Day 4 pancreatic duct IFL
m1018Tg; s854Tg standard conditions Day 4 gall bladder IFL
Day 4 pancreas IFL
Day 4 pancreatic duct IFL
RFP dnmt1s872/s872; gz15Tg standard conditions Day 4 endocrine pancreas pancreatic B cell IFL
Day 4 liver hepatocyte IFL
dnmt1s872/s872; gz15Tg; jh1Tg standard conditions Protruding-mouth endocrine pancreas pancreatic B cell IFL
Protruding-mouth liver hepatocyte IFL
Day 4 endocrine pancreas pancreatic B cell IFL
Day 4 liver hepatocyte IFL
Day 5 endocrine pancreas pancreatic B cell IFL
Day 5 liver hepatocyte IFL
Day 6 blood hepatocyte IFL
Day 6 endocrine pancreas pancreatic B cell IFL
Day 6 liver hepatocyte IFL
dnmt1s904/s904; gz15Tg standard conditions Day 4 endocrine pancreas pancreatic B cell IFL
Day 4 liver hepatocyte IFL
gz15Tg standard conditions Day 4 endocrine pancreas pancreatic B cell IFL
Day 4 liver hepatocyte IFL
gz15Tg; jh1Tg standard conditions Protruding-mouth endocrine pancreas pancreatic B cell IFL
Protruding-mouth liver hepatocyte IFL
Day 4 endocrine pancreas pancreatic B cell IFL
Day 4 liver hepatocyte IFL
Day 5 endocrine pancreas pancreatic B cell IFL
Day 5 liver hepatocyte IFL
Day 6 endocrine pancreas pancreatic B cell IFL
Day 6 liver hepatocyte IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-gcg IHC dnmt1s872/s872; m1018Tg; s854Tg standard conditions Day 4 pancreas glucagon secreting cell
IHC m1018Tg; s854Tg standard conditions Day 4 pancreas glucagon secreting cell
Ab2-isl IHC dnmt1s872/s872; m1018Tg standard conditions Day 4 endocrine pancreas pancreatic B cell
IHC m1018Tg standard conditions Day 4 endocrine pancreas pancreatic B cell
Ab2-nkx6 IHC dnmt1s872/s872; m1018Tg standard conditions Day 4 exocrine pancreas
IHC Day 4 pancreatic duct
IHC m1018Tg standard conditions Day 4 exocrine pancreas
IHC Day 4 pancreatic duct
ab2-sst IHC dnmt1s872/s872; m1018Tg standard conditions Day 4 pancreas somatostatin secreting cell
IHC m1018Tg standard conditions Day 4 pancreas somatostatin secreting cell
Phenotype Details
Fish Conditions Stage Phenotype
dnmt1s872/s872; gz15Tg standard conditions Day 4 exocrine pancreas aplastic, abnormal
Day 4 liver hypoplastic, abnormal
dnmt1s872/s872; gz15Tg; jh1Tg standard conditions Protruding-mouth exocrine pancreas hypoplastic, abnormal
Protruding-mouth liver hypoplastic, abnormal
Day 4 exocrine pancreas hypoplastic, abnormal
Day 4 liver hypoplastic, abnormal
Day 5 exocrine pancreas hypoplastic, abnormal
Day 5 liver hypoplastic, abnormal
Day 6 exocrine pancreas aplastic, abnormal
Day 6 liver hypoplastic, abnormal
dnmt1s872/s872; m1018Tg standard conditions Day 4 endocrine pancreas pancreatic B cell morphology, normal
Day 4 endocrine pancreas pancreatic B cell normal amount, normal
Day 4 endocrine pancreas somatostatin secreting cell functionality, normal
Day 4 pancreatic duct present, normal
dnmt1s872/s872; m1018Tg; s854Tg standard conditions Day 4 exocrine pancreas hypoplastic, abnormal
Day 4 pancreas pancreatic A cell normal amount, normal
dnmt1s904/s904; gz15Tg standard conditions Day 4 exocrine pancreas aplastic, abnormal
Day 4 liver hypoplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 334(1), Anderson, R.M., Bosch, J.A., Goll, M.G., Hesselson, D., Dong, P.D., Shin, D., Chi, N.C., Shin, C.H., Schlegel, A., Halpern, M., and Stainier, D.Y., Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration, 213-223, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.