FIGURE

Fig. 6

ID

ZDB-FIG-090714-15

Publication

Coffin et al., 2009 - Extracellular divalent cations modulate aminoglycoside-induced hair cell death in the zebrafish lateral line

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

GTTR uptake is substantially antagonized by external calcium and to a lesser degree by magnesium. Fish were incubated for 10 min in GTTR diluted in (A) 300 μM calcium, (B) 2100 μM calcium, (C) 300 μM magnesium, or (D) 2100 μM magnesium. (E) Negative control neuromast from a fish incubated with Texas Red only. GTTR fluorescence is red; nuclei are counter-stained blue with YO-PRO-1. GTTR fluorescence is greatly reduced in the presence of high calcium but there is little difference in fluorescence between the low and high magnesium conditions. The arrow in A indicates a fragmented hair cell. The scale bar in E is 5 μm and applies to all panels.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Hearing Research, 253(1-2), Coffin, A.B., Owens, K.N., Raible, D.W., and Rubel, E.W., Extracellular divalent cations modulate aminoglycoside-induced hair cell death in the zebrafish lateral line, 42-51, Copyright (2009) with permission from Elsevier. Full text @ Hear. Res.