ZFIN ID: ZDB-FIG-081222-3
Montpetit et al., 2008 - Disruption of AP1S1, causing a novel neurocutaneous syndrome, perturbs development of the skin and spinal cord. PLoS Genetics   4(12):e1000296 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 3 Ap1s1 knockdown is associated with abnormal distribution of laminin and cadherin.

Despite changes in the size and shape of the tail, p63-labelled nuclei (orange) of basal keratinocytes were present in KD larvae. (B). The insets show enlarged views of small groups of keratinocytes. Immunolabelling for laminin (green) in WT (C) showed normal distribution of the basement membrane along the fin fold margin, whereas in the KD larvae the residual laminin labeling was diffuse and disorganized (D). Compared to WT keratinocytes (E) labeled with p63 (orange), cadherin (green) is decreased at the cell membrane of the KD larvae (F). However, the localization of cytokeratin in WT (G) and in KD larvae (H) appeared similar. Scale bars: 10 μm, Insets in A, B, = 20 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-lama1 IHC WT control Long-pec caudal fin basement membrane
IHC WT + MO1-ap1s1 standard conditions Long-pec caudal fin basement membrane
Ab1-pan-Cadherin IHC WT control Long-pec caudal fin plasma membrane
IHC WT + MO1-ap1s1 standard conditions Long-pec caudal fin plasma membrane
Ab1-tp63 IHC WT control Long-pec keratinocyte
IHC WT + MO1-ap1s1 standard conditions Long-pec keratinocyte
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ap1s1 standard conditions Long-pec caudal fin basement membrane disorganized, abnormal
Long-pec caudal fin basement membrane distributed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.