Fig. 3

The Dorsal Longitudinal Vein supplies the mCP with its vascular network.
The Dorsal Longitudinal Vein (DLV) closely associates with the mCP epithelia as defined by doubly transgenic Et^Mn16/Tg(gata1:dsRed) dorsally-mounted 5 dpf live zebrafish, epithelia express GFP (A) and red blood cells express DsRed (B) merged image (C) (scale bars are 50 μm). The vessels, as defined by blood flow, directly contact the dorsal surface of the mCP. Low magnification of a 5 dpf dorsally mounted live Et^Mn16/Tg(gata1:dsRed), high magnification three-dimensional renders of DLV/PCeV junctions and TCB of zebrafish shown in D (E,F). Panel F is rotated 80° into the plane to show association (dorsal facing the top of the image). The DLV bends dorsally slightly before the bifurcation, traverses the mCP epithelial domain, and then turns ventrally as it transitions to the PCeV and PCeV′. Panels G and I are three-dimensional renders of the DLV, PCeV and TCB [(G,I – 85° rotation into the plane)(oriented with anterior to the right)]. The mCP has been digitally removed, and the DLV has been bisected utilizing the clipping tools of Image4D in order to define the vascular structure. Scale is as indicated on each render.