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ZIRC
ZFIN ID: ZDB-FIG-080829-47
Kishi et al., 2008 - The identification of zebrafish mutants showing alterations in senescence-associated biomarkers. PLoS Genetics   4(8):e1000152 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Pec-fin to Protruding-mouth

Fig. 3 Mutant nrs zebrafish show extremely high SA-β-gal activity and display both yolk and muscle phenotypes.
3.5-day old (3.5 dpf) homozygous nrsm/m (hi891/hi891) zebrafish embryos show extremely high SA-β-gal activity (B) compared with wild-type embryos (A) (with PTU). The atp6v1hm/m (hi923/hi923) mutant shown in (D) is another variant identified as having significantly higher SA-β-gal activity. Most other early embryonic lethal mutants derived from either insertional mutagenesis (as shown in Figure S4) and chemical mutagenesis (e.g., clom39/m39 which is shown in (C)) show no higher (or indistinguishable) SA-β-gal activity than wild-type siblings at any time during development. (E) Yolk opaque phenotypes can be observed in homozygous nrsm/m embryos at 3.5 dpf (earliest detection at around 2.5 dpf; lower left panel), compared with wild-type embryos (upper left panel). Also shown is a comparison between the H&E staining of transverse sections of the yolk part of nrsm/m embryos at 3.5 dpf (lower right panel) and wild-type embryos (upper right).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
atp6v1hhi923Tg/hi923Tg standard conditions Protruding-mouth cell aging increased magnitude, abnormal
npas4lDf(Chr13:lycat)m39/m39 standard conditions Protruding-mouth cell aging magnitude, normal
spns1hi891Tg/hi891Tg standard conditions Pec-fin - Protruding-mouth yolk opaque, abnormal
Protruding-mouth cell aging increased magnitude, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.