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ZFIN ID: ZDB-FIG-080730-15
Clément et al., 2008 - Regulation of zebrafish skeletogenesis by ext2/dackel and papst1/pinscher. PLoS Genetics   4(7):e1000136 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth

Fig. 2 Sulphated proteoglycans are reduced in dak-/- and pic-/- larvae.

Whole mount antibody staining at 24hpf reveals that HS is reduced in the somites of dak-/- and pic-/- (A–C) and KS in the notochord is reduced only in pic-/- larvae (D–F). Cartilage staining of the ceratohyal at 72hpf reveals that CS is made in wild-type and dak-/- chondrocytes (red stain in G,H), and absent from pic-/- cartilage (I) (nuclear staining is shown in blue with DAPI). HPLC analysis of HS in pic-/- larvae at day 5 indicates that sulphated disaccharides are nearly absent and unsulphated disaccharides are reduced (red) compared to their siblings (blue) (J). 1: ΔUA-GlcNAc, unsulfated Δ4,5-unsaturated hexuronate -N-acetyl glucosamine; 2: ΔUA-GlcNS, ΔUA-N-sulfated glucosamine; 3: ΔUA-GlcNAc6S, ΔUA-6-O-sulfated GlcNAc; 4: ΔUA-GlcNS6S, ΔUA-N-sulfated, 6-O-sulfated glucosamine; 5: ΔUA2S-GlcNS, 2-O-sulfated ΔUA-N-sulfated glucosamine; 6: ΔUA2S-GlcNS6S, 2-O-sulfated ΔUA-N-sulfated, 6-O-sulfated glucosamine. These disaccharides correspond to the major disaccharides found in both invertebrate and vertebrate animals. Panel F scale bar = 50μM. Panel I scale bar = 10μM.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-3H1 IHC WT standard conditions Prim-5 notochord
IHC ext2to273b/to273b standard conditions Prim-5 notochord
IHC slc35b2to14mx/to14mx standard conditions Prim-5 notochord
Ab-10E4 IHC WT standard conditions Prim-5 somite
IHC ext2to273b/to273b standard conditions Prim-5 somite
IHC slc35b2to14mx/to14mx standard conditions Prim-5 somite
Ab-CS-56 IHC WT standard conditions Protruding-mouth chondrocyte
IHC ext2to273b/to273b standard conditions Protruding-mouth chondrocyte
IHC slc35b2to14mx/to14mx standard conditions Protruding-mouth Not Detected chondrocyte
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.