FIGURE

Fig. S2

ID

ZDB-FIG-080604-77

Publication

Peri et al., 2008 - Live Imaging of Neuronal Degradation by Microglia Reveals a Role for v0-ATPase a1 in Phagosomal Fusion In Vivo

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Fig. S2

GT-MO injected embryos display a phenotype that is comparable with that observed for ATG-MO injected embryos
GT-MO morphant brain (A-C). APO-GFP (A), NBT.DsRed (B), merge (C). GT-MO injected embryos display a phenotype that is comparable with that observed for ATG-MO injected embryos (compare with Fig.4 L-O). Both are characterized an increase in the amount of NBT-DSRed neurons present in microglia (compare with Fig.4 H-K). D and E wild-type (D) and GT-MO (E) microglia stained with LysoSesor to label acidic vesicles. Intensity fluorescent values inside single vesicles (white arrowhead) are comparable, indicating normal acidification of these vesicles. (F) Efficacy of morpholino knock-down is confirmed by the 1.5kb size-shift in the transcript produced from the vAtpase gene (white arrowheads).

Expression Data

Genes:	DsRed EGFP
Fish:	zf147Tg; zf148Tg + MO2-atp6v0a1a
Knockdown Reagent:	MO2-atp6v0a1a
Anatomical Terms:	CNS neuron (sensu Vertebrata) microglial cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf147Tg; zf148Tg + MO2-atp6v0a1a
Knockdown Reagent:	MO2-atp6v0a1a
Observed In:	autophagosome maturation lysosomal lumen acidification phagolysosome assembly
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 133(5), Peri, F., and Nüsslein-Volhard, C., Live Imaging of Neuronal Degradation by Microglia Reveals a Role for v0-ATPase a1 in Phagosomal Fusion In Vivo, 916-927, Copyright (2008) with permission from Elsevier. Full text @ Cell