ZFIN ID: ZDB-FIG-080505-17
Matthews et al., 2008 - Directional migration of neural crest cells in vivo is regulated by Syndecan-4/Rac1 and non-canonical Wnt signaling/RhoA. Development (Cambridge, England)   135(10):1771-1780 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Day 5

Fig. 2 Syn4 is required for NC development but not for NC induction. (A-C) Efficiency of the syn4 MO. Zebrafish embryos were injected with 250 pg of syn4-egfp mRNA containing the MO-binding site (A). Fluorescence was significantly decreased by co-injection of 6 ng syn4 MO (B) and was totally abolished with 15 ng MO (C), demonstrating that the MO is able to efficiently inhibit Syn4. (D,E,G,H) Ventral view of zebrafish embryos showing cartilage development, analyzed by Alcian Blue staining (D,G) or cartilage fluorescence in the sox10:egfp line (E,H). (D,E) Control MO; (G) 6 ng of syn4 MO1; (H) 6 ng of syn4 MO2. (F,I) Lateral view of zebrafish embryos showing melanocytes. (F) Control MO; (I) syn4 MO. (J-O) Syn4 is not required for NC induction. (J) syn4 MO was co-injected with FDX into one blastomere of a two-cell stage Xenopus embryo and the expression of snail2 was analyzed at stage 18, before NC migration. No effect on the expression of snail2 was observed (96%, n=42). (K,L) Zebrafish embryos were injected with control MO (K) or syn4 MO (L), and the expression of the premigratory NC marker foxd3 was analyzed. No effect on the expression of foxd3 was produced by injection of syn4 MO. (M) syn4 MO was injected into one-cell stage Xenopus embryos, and at the blastula stage animal caps were dissected and conjugated with dorsolateral marginal zone (DLMZ), which is know to induce NC. The expression of snail2 was analyzed at the equivalent of stage 18. (N) Conjugates of DLMZ with control animal caps show a strong induction of snail2 (100%, n=15). (O) Conjugates of DLMZ with syn4-MO injected animal caps also show a strong induction of snail2 (100%, n=10).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ba2Tg control Day 5 cranial cartilage IFL
ba2Tg + MO2-sdc4 standard conditions Day 5 cranial cartilage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-sdc4 standard conditions Prim-5 melanocyte decreased amount, abnormal
Prim-5 melanocyte differentiation disrupted, abnormal
Day 5 cartilage development disrupted, abnormal
Day 5 cranial cartilage morphology, abnormal
Day 5 neural crest formation disrupted, abnormal
ba2Tg + MO2-sdc4 standard conditions Day 5 cartilage development disrupted, abnormal
Day 5 cranial cartilage morphology, abnormal
Day 5 neural crest formation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development