The development of lateral line was affected in MOstmie morphants. A-D: DASPEI staining of lateral line neuromasts. There is no or very little staining in MOstmie at 72 hpf (B) compared to the control (A) at the same stage. The lateral staining is weaker at 5 hpf in the MOstmie morphants (D) but present compared to the control larvae (C). E-L: Whole mount in situ hybridization with cxcr4b (E, F, I, J) and ath1 probes (G, H, K, L). Arrows, the migrating lateral line primordium. Arrowheads, the neuromasts with atoh1a signal. M: DASPEI score of the lateral line. After DASPEI staining, the larvae were divided into 3 groups: strongly stained, weakly stained, and not stained. Each fish was assigned a score after the level of staining in all the fish was first observed. Each larva in the strongly stained group was given 2 points, while 1 point was given to the weakly stained larvae, and 0 points to larvae, which were not stained. The number of fish in each group was recorded. The cumulative scores were then calculated and the percentage of fish in each group following each treatment determined. The higher the score (and the closer to 2.0), the stronger the staining of DASPEI observed after each treatment.
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