ZFIN ID: ZDB-FIG-080104-9
Shang et al., 2007 - The circadian system is a target and modulator of prenatal cocaine effects. PLoS One   2(1):e587 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage Range: Day 5 to Day 6

Fig. 4 Daily variation in melatonin receptor expression, cocaine efficacy and ability of melatonin to counteract the effects of cocaine are preserved during early postnatal period in zebrafish. (A) Normal daily pattern of mRNA expression for six melatonin receptors on Day 5, Night 5 and Day 6 post-fertilization in Control group, fold change expressed relative to Day 5 (=1). (B) Change in melatonin receptor expression following Daytime cocaine administration alone (Coc) or following melatonin pre-treatment (MLT+Coc) at 6 dpf, relative to Daytime Control group (=1); (C) Change in melatonin receptor expression following Nighttime cocaine administration alone (Coc) or following melatonin pre-treatment (MLT+Coc) at 6 dpf, relative to Nighttime Control group (=1). Cocaine (0.3 μM, 20 min), melatonin pre-treatment (100 nM, 30 min prior to cocaine). Y axis: fold change relative to corresponding control (=1). N=3–4 samples/treatment group, 20 larvae per sample. Mean±SEM, t-test (vs. control), * p<0.05, ** p<0.01, *** p<0.001 vs. cocaine-only group; One way ANOVA.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mtnr1aa AB chemical treatment: cocaine Day 5 to Day 6 whole organism RTPCR
AB chemical treatment: cocaine, chemical treatment: melatonin Day 5 to Day 6 whole organism RTPCR
mtnr1ab AB chemical treatment: cocaine Day 5 to Day 6 whole organism RTPCR
AB chemical treatment: cocaine, chemical treatment: melatonin Day 5 to Day 6 whole organism RTPCR
mtnr1al AB chemical treatment: cocaine Day 5 to Day 6 whole organism RTPCR
AB chemical treatment: cocaine, chemical treatment: melatonin Day 5 to Day 6 whole organism RTPCR
mtnr1ba AB chemical treatment: cocaine Day 5 to Day 6 whole organism RTPCR
AB chemical treatment: cocaine, chemical treatment: melatonin Day 5 to Day 6 whole organism RTPCR
mtnr1bb AB chemical treatment: cocaine Day 5 to Day 6 whole organism RTPCR
AB chemical treatment: cocaine, chemical treatment: melatonin Day 5 to Day 6 whole organism RTPCR
mtnr1c AB chemical treatment: cocaine Day 5 to Day 6 whole organism RTPCR
AB chemical treatment: cocaine, chemical treatment: melatonin Day 5 to Day 6 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One