ZFIN ID: ZDB-FIG-071116-22
Stellabotte et al., 2007 - Dynamic somite cell rearrangements lead to distinct waves of myotome growth. Development (Cambridge, England)   134(7):1253-1257 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5

Fig. 1 Distinct anterior and posterior domains in the epithelial somite. (A) In a 12S (15 hours) embryo, MyoD (red) is expressed in nuclei in the posterior of newly formed somites and in adaxial cells of the anterior segmental plate. (B) In the anterior of a 14S (16 hours) embryo, myogenin labeling (red) is in the posterior and medial somite, whereas Pax7 (green) is restricted to anterior and lateral. (C) In a 24-hour embryo, Pax7-labeled nuclei (green) are on the external myotome surface, whereas myogenin labeled nuclei (red) are more medial. (D) Bodipy ceramide-stained Histone H2:GFP 12S embryo. (E) Cells from the embryo in D are outlined, anterior border cells (ABCs) are marked by green dots. (F-K) Selected time-lapse images of green, GFP-labeled nuclei. T, time in minutes from the commencement of analysis. An individual ABC is pseudo-coloured green and tracked through individual frames. (K) An additional ABC is pseudo-coloured yellow in the same medio-lateral position as an elongating posterior cell in blue. (A-K) Dorsal views, anterior to the top, the midline is in the center in A-C, at the right in D-K. Scale bars: 50 μm in C for A-C, and 50 μm in D for D-K.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 Ab1-myod1 WT standard conditions 10-13 somites adaxial cell nucleus IHC
Ab1-myod1 10-13 somites somite nucleus IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-Pax7 IHC WT standard conditions 14-19 somites somite nucleus
IHC Prim-5 somite nucleus
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development