Fig. 7

Notch1 activity in peripheral nervous system (PNS) and sense organ development. (A-F) PNS, (G-P) inner ear, (Q-T) eye, (U-X) nose. A: General view of the central nervous system (CNS) at embryonic day (E) 10.5, at the level of the trigeminal ganglion. IV, fourth ventricle. Note strong N1ICD signal in ventricular zone (arrow). B: Detail showing N1ICD expression in trigeminal ganglion (V, arrow) and lack of signal in the primary head vein (phv, arrow). C,D: N1ICD staining in trigeminal ganglion at E11.5 with detail in (D). E,F: General view of spinal cord at E13.5, showing N1ICD expression in the ventricular zone (arrow) and dorsal root ganglia (DRG, F), where N1ICD-expressing cells (arrow) and nonexpressing cells are mixed (F). G,H: At E9.0, the invaginating otic cup (otc, arrow) expresses N1ICD. I,J: At E10.5, the ventral region of the otic vesicle (arrow) and cells of the acoustic ganglion complex (VIII) express N1ICD (arrows). K,L: At E11.5, N1ICD expression in the inner ear is restricted to specific cells of the endolymphatic sac (es, arrow), but the semicircular canal (scc) is negative. M: At E13.5, neuroepithelial cells in the cochlear canal (cc) express N1ICD (arrow), but the semicircular canal (thick arrow) remains negative. N: Higher magnification showing positive N1ICD staining in sensory cells of cochlea (arrow) and semicircular canal (thick arrows). O: General view of inner ear at E14.5. P: Detail showing N1ICD expression in neuroepithelium of the cochlear canal (arrow). Q: At E11.5, N1ICD staining is weakly expressed in the neural retina (nr, arrow). Signal is also detected in the optic stalk (os, arrow), hyaloid artery (ha, arrow) and vasculature around the eye. l, lens. R,S: At E13.5-E14.5, N1ICD staining is observed throughout the distal neural retina (arrow and inset in S) and hyaloid plexus (hp), but is excluded from the central retinal region (arrowheads). T: BrdU staining is restricted to the neural retina (arrow) and lens epithelium (thick arrow) and is excluded from central neural retina and lens fibers (arrowheads). U: General view of nasal pit region at E11.5. V: Detail showing N1ICD expression in nasal epithelium (arrow) and in surrounding vessels (arrowhead). W,X: General view of primitive nasal cavity at E13.5 (W) and detail showing N1ICD staining in serous gland (X, arrows). Scale bars = 600 μm in A,K,N, 250 μm in B,C,G,S,T, 150 μm in D, 1.2 mm in E, 350 μm in F, 80 μm in H, 300 μm in I,L, 150 μm in J, 800 μm in M, 1 mm in O, 500 μm in P, 200 μm in Q,R, 600 μm in U, 400 μm in V,W, 180 μm in X.