PUBLICATION
Monitoring Notch1 activity in development: Evidence for a feedback regulatory loop
- Authors
- Del Monte, G., Grego-Bessa, J., González-Rajal, A., Bolós, V., and De La Pompa, J.L.
- ID
- ZDB-PUB-070813-19
- Date
- 2007
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 236(9): 2594-2614 (Journal)
- Registered Authors
- de la Pompa, José Luis
- Keywords
- Notch, Val1744, N1ICD, cell fate specification, feedback loop, RBPJk, mind bomb, cardiovascular development, hematopoiesis, CNS development, somitogenesis, sense organs, thymus, pancreas
- MeSH Terms
-
- Animals
- Cardiovascular System/embryology
- Cell Lineage
- Central Nervous System/embryology
- Feedback, Physiological*
- Gene Expression Regulation, Developmental*
- Hematopoiesis
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/physiology*
- Mice
- Nerve Tissue Proteins/genetics*
- Nerve Tissue Proteins/physiology*
- Pancreas/embryology
- Receptor, Notch1/genetics*
- Receptor, Notch1/physiology*
- Receptors, Notch/metabolism
- Thymus Gland/embryology
- Tissue Distribution
- Zebrafish
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/physiology*
- PubMed
- 17685488 Full text @ Dev. Dyn.
Citation
Del Monte, G., Grego-Bessa, J., González-Rajal, A., Bolós, V., and De La Pompa, J.L. (2007) Monitoring Notch1 activity in development: Evidence for a feedback regulatory loop. Developmental Dynamics : an official publication of the American Association of Anatomists. 236(9):2594-2614.
Abstract
Signaling through Notch receptors, which regulate cell fate decisions and embryonic patterning, requires ligand-induced receptor cleavage to generate the signaling active Notch intracellular domain (NICD). Here, we show an analysis at specific developmental stages of the distribution of active mouse Notch1. We use an antibody that recognizes N1ICD, and a highly sensitive staining technique. The earliest N1ICD expression was observed in the mesoderm and developing heart, where we detected expression in nascent endocardium, presumptive cardiac valves, and ventricular and atrial endocardium. During segmentation, N1ICD was restricted to the presomitic mesoderm. N1ICD expression was also evident in arterial endothelium, and in kidney and endodermal derivatives such as pancreas and thymus. Ectodermal N1ICD expression was found in central nervous system and sensory placodes. We found that Notch1 transcription and activity was severely reduced in zebrafish and mouse Notch pathway mutants, suggesting that vertebrate Notch1 expression is regulated by a positive feedback loop.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping