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ZIRC
ZFIN ID: ZDB-FIG-070314-17
Leslie et al., 2007 - Endothelial signalling by the Notch ligand Delta-like 4 restricts angiogenesis. Development (Cambridge, England)   134(5):839-844 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage Range: Pec-fin to Protruding-mouth

Fig. 2 Effects of Dll4 knockdown on circulation and vascular pattern. (A) Blood flow in intersegmental vessels (ISVs) of wild-type living embryos at 3 dpf. Each line represents a set of ISVs on one side of one embryo, scored for direction of blood flow: upward arrows (red) denote flow from ventral to dorsal; downward arrows (blue), denote flow from dorsal to ventral; dots represent vessels carrying no flow. (B,C) Blood flow in intersegmental vessels of living embryos at 3 dpf injected with either 10 ng MO[Dll4] to knockdown Dll4 (B) or with 10 ng of a 5-base mismatch control morpholino (C). (D-G) fli1:EGFP embryos at 2.5 dpf (D,E) or 5 dpf (F,G) either uninjected (D,F) or injected with 10 ng MO[Dll4] (E,G). White blobs (arrows in D,E) are endothelial cell nuclei. (H) Similar region of a 2.5-dpf dll4-homozygous-mutant embryo (carrying the fli1:EGFP transgene). (I) Endothelial cell counts in the DAs, ISVs and DLAVs of embryos at 3 dpf. Embryos lacking Dll4 or treated with 100 μm DAPT have more cells than uninjected or DMSO-treated control siblings. Both effects are significant at the P=0.001 level (t-test; n≥6 specimens for each treatment; error bars represent s.e.m.). (J) fli1:EGFP embryo at 2.5 dpf injected with 10 ng of a morpholino targeted against casanova (sox32) to block circulation in the trunk. (K) fli1:EGFP-positive dll4-mutant embryo at 2.5 dpf treated from 48 hpf with 2 μM SU5416 to block Vegf signalling. (L) The gut and pectoral fin vasculature of an fli1:EGFP-positive dll4 mutant and a wild-type (normal) sibling embryo, both at 11 dpf. Scale bar: 50 μm in D-H,J,K; 40 μm in L.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: DAPT Protruding-mouth blood vessel endothelial cell increased amount, abnormal
WT + MO3-dll4 standard conditions Protruding-mouth blood vessel endothelial cell increased amount, abnormal
dll4j16e1/j16e1; y1Tg standard conditions Pec-fin blood circulation decreased rate, abnormal
Pec-fin blood vessel morphogenesis disrupted, abnormal
Pec-fin dorsal aorta morphology, abnormal
Pec-fin gut blood vasculature increased amount, abnormal
Pec-fin intersegmental vessel morphology, abnormal
Pec-fin pectoral fin blood vasculature increased amount, abnormal
Pec-fin posterior cardinal vein morphology, abnormal
y1Tg chemical treatment: semaxanib Pec-fin sprouting angiogenesis disrupted, abnormal
y1Tg + MO1-sox32 standard conditions Pec-fin blood circulation decreased rate, abnormal
y1Tg + MO3-dll4 standard conditions Pec-fin - Protruding-mouth blood circulation decreased rate, abnormal
Pec-fin - Protruding-mouth dorsal aorta morphology, abnormal
Pec-fin - Protruding-mouth intersegmental vessel morphology, abnormal
Pec-fin - Protruding-mouth posterior cardinal vein morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development