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ZIRC
ZFIN ID: ZDB-FIG-051228-11
Ebert et al., 2005 - Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts. Proceedings of the National Academy of Sciences of the United States of America   102(49):17705-17710 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 4

SERCA2 is essential for cardiac development. (A–F) SERCA2 expression in early development. SERCA2 RNA is expressed in (A–C) the bilateral cardiac precursors; (D) the heart tube at 26 hpf; (E) the somites and mesenchyme of the fin buds (arrows); and (F) the facial, jaw, and pectoral fin muscles at 72 hpf. (G–K2) Comparison of SERCA2 expression in wild-type (G–K) and tre mutants (G2–K2). (G and G2) At 30 hpf, SERCA2 expression in tre heart tubes is comparable to wild type. However, at (H and H2) 48 hpf, (J and J2) 53 and 72 hpf (not shown) wild-type embryos show diffuse expression of SERCA2 in the atrium, whereas tre mutants show robust expression of SERCA2 in the atrium. At (I and I2) 48 hpf and (K and K2) 53 and 72 hpf (not shown), probes for pancardiac genes cmlc2 (and tbx20, not shown) exhibit the usual diffuse signal in the atrium for both wild-type and tre embryos, indicating robust expression of SERCA2 in tre mutants is not an artifact of morphology. (L–T) SERCA2 knockdown phenotypes. Embryos injected with 17 ng of (M, P, and S) SERCA2 morpholino (MO2) or (Q and T)5 µM CPA have heart rates H30–50% slower than normal. Hearts show weak contractility in both chambers and fail to loop but no fibrillation or arrhythmia (other than heart rate). Most fail to circulate blood. SERCA2-inhibited embryos are touch-insensitive although not completely paralyzed. a, atrium; v, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atp2a2a WT standard conditions 10-13 somites heart primordium ISH
20-25 somites heart primordium ISH
Prim-5 heart tube ISH
Prim-15 heart tube ISH
Long-pec atrium ISH
Long-pec cardiac ventricle ISH
Long-pec pectoral fin ISH
Long-pec somite ISH
Protruding-mouth cardiac ventricle ISH
Protruding-mouth cephalic musculature ISH
Protruding-mouth pectoral fin musculature ISH
slc8a1am139/m139(AB) standard conditions Prim-15 heart tube ISH
Long-pec atrium ISH
Long-pec cardiac ventricle ISH
myl7 WT standard conditions Long-pec atrium ISH
Long-pec cardiac ventricle ISH
slc8a1am139/m139(AB) standard conditions Long-pec atrium ISH
Long-pec cardiac ventricle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-atp2a2a standard conditions Long-pec atrium collapsed, abnormal
Long-pec atrium decreased contractility, abnormal
Long-pec cardiac ventricle collapsed, abnormal
Long-pec cardiac ventricle decreased contractility, abnormal
Long-pec heart contraction decreased rate, abnormal
Long-pec heart looping arrested, abnormal
Long-pec pericardium edematous, abnormal
Long-pec sensory perception of touch disrupted, abnormal
Protruding-mouth atrium collapsed, abnormal
Protruding-mouth atrium decreased contractility, abnormal
Protruding-mouth cardiac ventricle collapsed, abnormal
Protruding-mouth cardiac ventricle decreased contractility, abnormal
Protruding-mouth heart contraction decreased rate, abnormal
Protruding-mouth heart looping arrested, abnormal
Protruding-mouth pericardium edematous, abnormal
Protruding-mouth sensory perception of touch disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA