header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-051004-5
Feng et al., 2005 - The recombination activation gene 1 (Rag1) is expressed in a subset of zebrafish olfactory neurons but is not essential for axon targeting or amino acid detection. BMC Neuroscience   6:46 Full text @ BMC Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 5 The effect of RAG1 depletion on the olfactory projection. (A) An embryo injected with mRNA encoding the 5' end of Rag1 fused to EGFP. (B) An embryo co-injected with the Rag1-EGFP fusion mRNA and mo1. (C) Olfactory neurons labelled with GFP under the Rag1 promoter, with brightly labeled axons projecting to a single target (arrowhead), at 3 dpf. (D) In a transgenic embryo injected with mo1, axons still project to the same target (arrowhead), but the intensity of GFP fluorescence is reduced. (E) A schematic diagram of the Rag1 gene, showing the location of morpholinos and primers that were used to analyse morpholino-injected fish. (F) RT-PCR on control or mo2 injected embryos. Abnormal splicing occurs in the morpholino-injected fish, leading to a premature stop codon, as indicated by sequencing of the upper band. (G) RT-PCR after injection of a mixture of Rag1 mo2 and mo3, showing loss of the normal transcript. (H, I) A subset of Di8ANEPPQ-labeled olfactory sensory neurons in 7-day old wild type and Rag1 mutant fish. Axons innervate all target structures detectable in this optical plane in the mutant. (J-L) SV2-labelled 4 day-old Rag1:GFP transgenic (J, K) and Rag1 mutant (L) forebrains, shown in dorsal view. The images are colour-coded according to depth. The glomerulus innervated by the strong GFP-positive neurons (J) is indicated by the arrowhead. Bar = 100 µm (A, B); 20 µm (C, D, H-K). The colour bars in J and K indicate depth. Embryos in panels H-L are shown in dorsal view, with anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP la5Tg standard conditions Day 4 olfactory bulb glomerulus IHC
rag1t26683/t26683; la5Tg standard conditions Day 4 olfactory bulb glomerulus IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-SV2 IHC la5Tg standard conditions Day 4 forebrain
IHC rag1t26683/t26683; la5Tg standard conditions Day 4 forebrain
Phenotype Details
Fish Conditions Stage Phenotype
rag1t26683/t26683 standard conditions Days 7-13 lateral zone olfactory bulb morphology, normal
Days 7-13 sensory neuron axon guidance process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal BMC Neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Neurosci.