CRISPR

CRISPR1-hes4

ID

ZDB-CRISPR-210721-2

Name

CRISPR1-hes4

Previous Names

CRISPR1-her9

Target

hes4 (1)

Sequence

5' - GTATGAGATCCACTGGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGA" at the 5' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
uky2	hes4
uky3	hes4

Expression

Gene expression in Wild Types + CRISPR1-hes4

No data available

Phenotype

Phenotype resulting from CRISPR1-hes4

No data available

Phenotype of all Fish created by or utilizing CRISPR1-hes4

Phenotype	Fish	Conditions	Figures
retina ventral region thrb expression decreased amount, abnormal	hes4^uky2/uky2	standard conditions	Figure 10 from Coomer et al., 2020
retinal cone cell cone photoreceptor outer segment decreased length, abnormal	hes4^uky2/uky2	standard conditions	Figure 5 from Coomer et al., 2020
retinal cone cell decreased amount, abnormal	hes4^uky2/uky2	standard conditions	Figure 5 from Coomer et al., 2020
retinal ganglion cell layer apoptotic process increased occurrence, abnormal	hes4^uky2/uky2	standard conditions	Figure 11 from Coomer et al., 2020
retinal rod cell decreased amount, abnormal	hes4^uky2/uky2	standard conditions	Figure 4 from Coomer et al., 2020
whole organism decreased pigmentation, abnormal	hes4^uky2/uky2	standard conditions	Figure 2 from Coomer et al., 2020
optomotor response decreased process quality, abnormal	hes4^uky2/uky2	standard conditions	Figure 3 from Coomer et al., 2020
retinal cone cell fate specification process quality, abnormal	hes4^uky2/uky2	standard conditions	Figure 10 from Coomer et al., 2020
visual system decreased functionality, abnormal	hes4^uky2/uky2	standard conditions	Figure 3 from Coomer et al., 2020
ciliary marginal zone decreased size, abnormal	hes4^uky2/uky2	standard conditions	Figure 9 from Coomer et al., 2020
visually-mediated background adaptation decreased process quality, abnormal	hes4^uky2/uky2	standard conditions	Figure 3 from Coomer et al., 2020
retinal inner nuclear layer apoptotic process increased occurrence, abnormal	hes4^uky2/uky2	standard conditions	Figure 11 from Coomer et al., 2020
whole organism dead, abnormal	hes4^uky2/uky2	standard conditions	Figure 2 from Coomer et al., 2020
eye decreased size, abnormal	hes4^uky2/uky2	standard conditions	Figure 2 from Coomer et al., 2020
whole organism decreased size, abnormal	hes4^uky2/uky2	standard conditions	Figure 2 from Coomer et al., 2020
pericardium edematous, abnormal	hes4^uky2/uky2	standard conditions	Figure 2 from Coomer et al., 2020
swim bladder aplastic, abnormal	hes4^uky2/uky2	standard conditions	Figure 2 from Coomer et al., 2020
retinal rod cell rod photoreceptor outer segment morphology, abnormal	hes4^uky2/uky2	standard conditions	Figure 4 from Coomer et al., 2020
ciliary marginal zone apoptotic process increased occurrence, abnormal	hes4^uky2/uky2	standard conditions	Figure 11 from Coomer et al., 2020
retinal ganglion cell decreased amount, abnormal	hes4^uky2/uky2	standard conditions	Figure 8 from Coomer et al., 2020
whole organism viability, abnormal	hes4^uky2/uky2	standard conditions	Figure 2 from Coomer et al., 2020
retinal outer nuclear layer thrb expression decreased amount, abnormal	hes4^uky2/uky2	standard conditions	Figure 10 from Coomer et al., 2020
retinal outer nuclear layer apoptotic process increased occurrence, abnormal	hes4^uky2/uky2	standard conditions	Figure 11 from Coomer et al., 2020
retinal outer nuclear layer nr2e3 expression decreased amount, abnormal	hes4^uky2/uky2	standard conditions	Figure 10 from Coomer et al., 2020
whole organism increased curvature, abnormal	hes4^uky2/uky2	standard conditions	Figure 2 from Coomer et al., 2020
cranial skeletal system development disrupted, abnormal	hes4^uky2/uky2	standard conditions	Figure 2 from Coomer et al., 2020
retina thrb expression spatial pattern, abnormal	hes4^uky2/uky2	standard conditions	Figure 10 from Coomer et al., 2020
retina crx expression decreased amount, abnormal	hes4^uky2/uky2	standard conditions	Figure 10 from Coomer et al., 2020
liver increased size, abnormal	hes4^uky2/uky2	standard conditions	Figure 2 from Coomer et al., 2020
whole organism thrb expression decreased amount, abnormal	hes4^uky2/uky2	standard conditions	Figure 10 from Coomer et al., 2020
retinal cone cell decreased amount, abnormal	hes4^uky3/uky3	standard conditions	Figure S1 from Coomer et al., 2020
retinal rod cell decreased amount, abnormal	hes4^uky2/uky2; fl1Tg	control	Figure 4 , Figure 12 from Coomer et al., 2020
retinal rod cell amount, ameliorated	hes4^uky2/uky2; fl1Tg	chemical treatment by environment: all-trans-retinoic acid	Figure 12 from Coomer et al., 2020
retinal rod cell rod photoreceptor outer segment morphology, abnormal	hes4^uky2/uky2; fl1Tg	standard conditions	Figure 4 from Coomer et al., 2020
Muller cell glial cell projection decreased amount, abnormal	hes4^uky2/uky2; mi2001Tg	standard conditions	Figure 7 from Coomer et al., 2020
Muller cell disorganized, abnormal	hes4^uky2/uky2; mi2001Tg	standard conditions	Figure 7 from Coomer et al., 2020
Muller cell cell body position, abnormal	hes4^uky2/uky2; mi2001Tg	standard conditions	Figure 7 from Coomer et al., 2020
enteric nervous system glial cell decreased amount, abnormal	hes4^uky2/uky2; mi2001Tg	standard conditions	Figure S4 from Coomer et al., 2020
brain glial cell decreased amount, abnormal	hes4^uky2/uky2; mi2001Tg	standard conditions	Figure S4 from Coomer et al., 2020
Muller cell decreased amount, abnormal	hes4^uky2/uky2; mi2001Tg	standard conditions	Figure 7 from Coomer et al., 2020
long double cone cell cone photoreceptor outer segment morphology, abnormal	hes4^uky2/uky2; ucd1Tg	standard conditions	Figure 5 from Coomer et al., 2020
retinal cone cell decreased amount, exacerbated	hes4^uky2/uky2; ucd1Tg	chemical treatment by environment: all-trans-retinoic acid	Figure 12 from Coomer et al., 2020
retinal cone cell decreased amount, abnormal	hes4^uky2/uky2; ucd1Tg	control	Figure 5 , Figure 12 from Coomer et al., 2020
short single cone cell decreased amount, abnormal	hes4^uky2/uky2; ucd1Tg	control	Figure 12 from Coomer et al., 2020
short double cone cell cone photoreceptor outer segment morphology, abnormal	hes4^uky2/uky2; ucd1Tg	standard conditions	Figure 5 from Coomer et al., 2020

Citations