FIGURE

Figure 8

ID
ZDB-FIG-200713-26
Publication
Coomer et al., 2020 - Her9/Hes4 is required for retinal photoreceptor development, maintenance, and survival
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Figure 8

Loss of her9 has minimal effects other retinal cell types. Whole eye images of WT and heterozygous embryos (A) or her9 mutant embryos (B) on the Ath5:GFP transgenic background at 5 dpf. Immunohistochemistry for ganglion and amacrine cells (HuC/D antibody) in cryosections from WT and heterozygous retinas (CD) or her9 mutant retinas (EF). Ganglion cell: WT/Het = 10 embryos; Mut = 10 embryos; t-test (p < .0001). Horizontal cells: WT/Het = 10 embryos; Mut = 10 embryos; t-test (p = .455). Amacrine cells: WT/Het = 10 embryos; Mut = 10 embryos; t-test (p = 0.5). (GH′) Immunohistochemistry with the Prox1 antibody labels horizontal cells in cryosections of WT and heterozygous (GH′) or her9 mutant (IJ′) retinas. Cell counts reveal a decrease in the number of ganglion cells in the mutant retinas (K) but no significant difference in the number of horizontal or amacrine cells (L-M). Am, Amacrine cells; GC, Ganglion cells; Hz, Horizontal cells; ONL, outer nuclear layer; INL, inner nuclear layer; ON, optic nerve; L, Lens. Scale bar = 50 µm and 100 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Day 5

Phenotype Detail
Acknowledgments
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