FIGURE SUMMARY
Title

Multi-omics association pattern between gut microbiota and host metabolism of a filter-feeding fish in situ exposed to microplastics

Authors
Huang, J.N., Gao, C.C., Ren, H.Y., Wen, B., Wang, Z.N., Gao, J.Z., Chen, Z.Z.
Source
Full text @ Environ. Int.
Fig. 1

Bacterial community composition in the gut of silver carp after three-month in situ MPs exposure. Different lowercase letters indicate significant differences at p < 0.05. (A) Alpha diversity. (B) PCoA at OTU level. (C) Relative abundance of phyla. (D) Phyla with significant differences. (E) The top 5 genera with significant differences.

Fig. 2

Liver transcriptome analysis of silver carp after three-month in situ MPs exposure. (A) PLS-DA of overall transcript profiles for three groups. (B) Volcano plot of DEGs between MPs-exposed groups and control. (C) KEGG functional enrichment analysis based on DEGs. (D) Expression levels of DEGs in the top five significant enrichment pathways obtained from Fig. 2C.

Fig. 3

WGCNA analysis between gut microbiota and liver transcriptome of silver carp after three-month in situ MPs exposure. (A) Correlation heatmap between differential gut phyla and liver gene modules (ME). (B) KEGG functional enrichment analysis based on key gene module MEdarkgreen.

Fig. 4

Transcriptome analysis of germ free (GF) zebrafish transplanted by gut microbiota of MPs-exposed silver carp. (A) PLS-DA of overall transcript profiles for three groups. (B) Correlation heatmap between differential gut phyla of silver carp and gene modules (ME) of GF zebrafish by WGCNA. (C) KEGG functional enrichment analysis based on key gene module MEred. (D) Expression levels of shared DEGs in the gene module MEdarkgreen of silver carp and MEred of GF zebrafish.

Fig. 5

Liver metabolome analysis of silver carp after three-month in situ MPs exposure. (A) PLS-DA of overall metabolite profiles for three groups. (B) Volcano plot of DEMs between MPs-exposed groups and control. (C) KEGG topological analysis based on DEMs. (D) Expression levels of DEMs in the top five significant enrichment pathways obtained from Fig. 4C. Different lowercase letters indicate significant differences at p < 0.05.

Fig. 6

Muscle metabolome analysis of silver carp after three-month in situ MPs exposure. (A) PLS-DA of overall metabolite profiles for three groups. (B) Volcano plot of DEMs between MPs-exposed groups and control. (C) KEGG topological analysis based on DEMs.

Fig. 7

Multi-omics joint analysis of silver carp after three-month in situ MPs exposure. (A) The shared pathway enriched with DEGs and DEMs in liver. (B) Relevance network constructed from the correlation matrix to identify the association between liver DEMs and muscle DEMs. Based on the inferred correlation matrix, variables exceeding a correlation coefficient threshold of (r = 0.9) are considered highly associated. (C) A conceptual model of MPs interfering with the metabolism of silver carp through the gut-liver-muscle axis.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Environ. Int.
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.