Zebrafish larvae whole body glucose and insulin. (A) Whole body glucose measurements of abcc8+/+ control (95% CI 259.4, 294.2) and abcc8-/- (95% CI 226.9, 259.3) larvae (5 days post-fertilization (dpf)) (p=5.5×10-3, n=100). (B) Evaluation of whole body insulin in abcc8+/+ control (95% CI 0.009, 0.01) and abcc8-/- (95% CI 0.013, 0.014) zebrafish larvae (5 dpf) (p=4.4×10-11, n=100). **p=5.5×10-3; ***p=4.4×10-11. Data represent mean±SEM

Zebrafish larvae pancreatic islet cytoplasmic Ca2+ measurements. (A) Measurement of zebrafish pancreatic islet cytosolic Ca2+ fluorescence in abcc8+/+ control (95% CI 8.4×104, 8.8×104) and abcc8-/- (95% CI 1.1×105, 1.2×105) zebrafish larvae (5 dpf) (p=0.0216, n=8–10). (B) Zebrafish larvae pancreatic islet cytosolic Ca2+ measurements in response to glucose stimulation in abcc8-/- (95% CI 1.6×105, 1.7×105) and control abcc8+/+ (95 % CI 1.4×105, 1.6×105) larvae (p=0.51, n=8–10). (C) Assessment of zebrafish larvae pancreatic islet cytosolic Ca2+ in response to glutamine in abcc8+/+ control (95% CI 9.7×104, 1×105), and abcc8-/- (95% CI 1.32×105, 1.38×105) larvae (p=0.0486, n=8–10). (D) Area under the curve calculations for cytoplasmic Ca2+ experiments as denoted. *p<0.05. Data represent mean±SEM.

Evaluation of adult zebrafish plasma glucose, insulin, and glucose tolerance. (A) Plasma glucose measurements in adult abcc8+/+ control (95% CI 58, 66) and abcc8-/- (95% CI 63, 75) zebrafish (p=0.059, n=123). (B) Plasma insulin values in adult abcc8+/+ control (95% CI 0.88, 1.49) and abcc8-/- (95% CI 0.91, 1.58) zebrafish (p=0.777, n=13–14 pools). (C) Insulin to glucose ratios in adult abcc8+/+ control (95% CI 0.32, 0.72) and abcc8-/- (95% CI 0.40, 0.97) zebrafish (p=0.3, n=13–14 pools). (D) Glucose tolerance test (GTT) in adult abcc8+/+ control (n=10 zebrafish per time point) and abcc8-/- zebrafish (n=9 zebrafish per timepoint). (E) Area under the curve (AUC) calculations of the GTT in adult zebrafish (p=0.44 comparing abcc8+/+ glucose vs abcc8-/- glucose). Data represent mean±SEM.