FIGURE SUMMARY
Title

Protocol to evaluate hyperlipidemia in zebrafish larvae

Authors
Balamurugan, K., Medishetti, R., Rao, P., K, R.V., Chatti, K., Parsa, K.V.L.
Source
Full text @ STAR Protoc
Fig. 1

Oil Red O and Nile red staining protocol of HFD-fed zebrafish larvae

Fig. 2

Comparative analysis of Oil Red O versus Nile red staining and flowchart of ImageJ quantification of inter-segmental vessels in HFD-fed zebrafish larvae

(A) Oil Red O and Nile red staining of zebrafish larvae fed with control diet (CD) or High Fat Diet (HFD) for 7 or 13 days.

(B) Nile red staining of zebrafish larvae at different light intensities. Left panels: control diet; Right Panels: HFD-fed.

(C) Workflow for ImageJ based quantification of lipid accmulation in the inter-segmental vessels (ISV) of HFD-fed zebrafish larvae.
Fig. 3

Larval homogenization, schematic representation of lipid extraction and cholesterol/free fatty acid analysis in zebrafish larval model of lipid accumulation

(A) Picture guided workflow for the homogenization of zebrafish larvae.

(B) Flowchart depiciting the steps involved in lipid extraction and analysis of cholesterol and free fatty acids in zebrafish larvae.
Fig. 4

Pictorial depiction of cholesterol and free fatty acid estimation in HFD-fed zebrafish larvae

Fig. 5

HPLC chromatograms of cholesterol standards and 15 dpf zebrafish larval samples

Fig. 6

Flowchart for ImageJ-based length and width measurement of zebrafish larvae

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ STAR Protoc
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.