PUBLICATION
Protocol to evaluate hyperlipidemia in zebrafish larvae
- Authors
- Balamurugan, K., Medishetti, R., Rao, P., K, R.V., Chatti, K., Parsa, K.V.L.
- ID
- ZDB-PUB-221118-13
- Date
- 2022
- Source
- STAR protocols 3: 101819 (Journal)
- Registered Authors
- Chatti, Kiranam
- Keywords
- Metabolism, Model organisms
- MeSH Terms
-
- Animals
- Hypercholesterolemia*/genetics
- Hyperlipidemias*/diagnosis
- Larva
- Lipids
- Zebrafish
- PubMed
- 36386866 Full text @ STAR Protoc
Citation
Balamurugan, K., Medishetti, R., Rao, P., K, R.V., Chatti, K., Parsa, K.V.L. (2022) Protocol to evaluate hyperlipidemia in zebrafish larvae. STAR protocols. 3:101819.
Abstract
In this report, we describe an approach to generate a zebrafish larval model of lipid accumulation that can be used as an in vivo system to study hyperlipidemic conditions such as atherosclerosis. Furthermore, we detail steps on staining techniques, lipid estimation assays, RNA isolation, and utilization of ImageJ to evaluate larval dimensions and to explore the model in the context of hyperlipidemia. Researchers should be aware of context specificity of the proposed protocols and interpret results accordingly. For complete details on the use and execution of this protocol, please refer to Balamurugan et al., (2022).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping