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Fig. 2 Comparative analysis of Oil Red O versus Nile red staining and flowchart of ImageJ quantification of inter-segmental vessels in HFD-fed zebrafish larvae
(A) Oil Red O and Nile red staining of zebrafish larvae fed with control diet (CD) or High Fat Diet (HFD) for 7 or 13 days.
(B) Nile red staining of zebrafish larvae at different light intensities. Left panels: control diet; Right Panels: HFD-fed.
(C) Workflow for ImageJ based quantification of lipid accmulation in the inter-segmental vessels (ISV) of HFD-fed zebrafish larvae.
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