- Title
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Comparative transcriptome analysis of heat-induced domesticated zebrafish during gonadal differentiation
- Authors
- Wang, C., Chen, X., Dai, Y., Zhang, Y., Sun, Y., Cui, X.
- Source
- Full text @ BMC Genom Data
Early treatment (25–35 dpf) with high temperature (35 °C) increased the proportion of males. The mean ± SEM of three biological replicates for control (28 °C; n = 95) and treated group (35 °C; n = 104) is shown. Statistically significant differences were found between the two sex ratios (***P < 0.001; T-test) |
Phenotypic identification and analysis of temperature-treated fish. (a) the comparative analysis of GSI between high temperature (35 °C) and normal temperature (28 °C) (T-test, *P < 0.05). (b) Histological analysis of gonad tissue. Stage IA and stage IB, primary growth stage; stage II, cortial alveolus stage; stage III, vitellogenesis stage; SC, spermatocytes; SG, spermatogonia; SZ, spermatozoa |
Analysis of DEGs between high temperature and normal temperature treatments at 35dpf, 45dpf and 60dpf. Number of DEGs identified from each comparison groups (a), shown by Venn diagram (b), and list of pathways [29, 30] involved in the gonad development in juvenile fish (c) |