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Fig. 1
Early treatment (25–35 dpf) with high temperature (35 °C) increased the proportion of males. The mean ± SEM of three biological replicates for control (28 °C; n = 95) and treated group (35 °C; n = 104) is shown. Statistically significant differences were found between the two sex ratios (***P < 0.001; T-test)
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