The free radical scavenging effects of MZPS-2 in zebrafish larvae. (a) Fluorescence micrographs of Tg(krt4:NTR-hKitGR)cy17 zebrafish (magnification 40x); (b) The number of fluorescent dots in zebrafish epidermal cells. VC: vehicle control group (fish water); MC: model control group (fish water containing metronidazole); PC: positive control group (vitamin C-treated group); I, II, and III: three MZPS-2-treated groups (10, 25, and 50 μg/mL, respectively); IV, V, and VI: three MPS-2-treated groups (10, 25, and 50 μg/mL, respectively). ^∗P < 0.05, ^∗∗P < 0.01 versus MC; ^##P < 0.01 versus VC. Data are presented as means ± SD (n =10).

Activities of MZPS-2 on LPS-stimulated ROS generation in zebrafish larvae. (a) Fluorescence micrographs of ROS generation in zebrafish larvae (magnification 40x). (b) Quantitative analysis of ROS generation. ROS level was measured after staining with DCF-DA. VC: vehicle control group (fish water); MC: model control group (fish water containing LPS); I, II, and III: three MZPS-2-treated groups (10, 25, and 50 μg/mL, respectively); IV, V, and VI: three MPS-2-treated groups (10, 25, and 50 μg/mL, respectively). ^∗P < 0.05, ^∗∗P < 0.01 versus MC; ^##P < 0.01 versus VC; ^aP < 0.05 versus the other group. Data are presented as means ± SD (n = 10).

Anti-inflammatory activities of MZPS-2 on neutrophil migration in zebrafish larvae. (a) Fluorescence micrographs of neutrophil migration in Tg(Lyz:GFP) zebrafish (magnification 40x). (b) Quantitative analysis of neutrophil migration. VC: vehicle control group (fish water); MC: model control group (fish water containing CuSO₄); PC: positive control group (indomethacin-treated group); I, II, and III: three MZPS-2-treated groups (10, 25, and 50 μg/mL, respectively); IV, V, and VI: three MPS-2-treated groups (10, 25, and 50 μg/mL, respectively). ^∗P < 0.05, ^∗∗P < 0.01 versus MC; ^##P < 0.01 versus VC. The neutrophils infiltrated into the lateral line were indicated by white arrowheads. Data are presented as means ± SD (n = 10).