PUBLICATION
Distribution of Zinc in Mycelial Cells and Antioxidant and Anti-Inflammatory Activities of Mycelia Zinc Polysaccharides from Thelephora ganbajun TG-01
- Authors
- Zheng, L., Ma, Y., Zhang, Y., Meng, Q., Yang, J., Gong, W., Liu, Q., Cai, L., Hao, L., Wang, B., Yang, Y.
- ID
- ZDB-PUB-200715-5
- Date
- 2020
- Source
- Oxidative medicine and cellular longevity 2020: 2308017 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/isolation & purification
- Anti-Inflammatory Agents/pharmacology*
- Antioxidants/isolation & purification
- Antioxidants/metabolism
- Antioxidants/pharmacology*
- Basidiomycota/metabolism*
- Carbohydrate Conformation
- Fungal Polysaccharides/chemistry
- Fungal Polysaccharides/metabolism
- Fungal Polysaccharides/pharmacology*
- Molecular Weight
- Monosaccharides/chemistry
- Mycelium/metabolism
- Neutrophil Infiltration/drug effects
- Oxidative Stress/drug effects
- Reactive Oxygen Species/metabolism
- Zebrafish
- Zinc/chemistry
- Zinc/metabolism*
- Zinc/pharmacology
- PubMed
- 32655762 Full text @ Oxid Med Cell Longev
Citation
Zheng, L., Ma, Y., Zhang, Y., Meng, Q., Yang, J., Gong, W., Liu, Q., Cai, L., Hao, L., Wang, B., Yang, Y. (2020) Distribution of Zinc in Mycelial Cells and Antioxidant and Anti-Inflammatory Activities of Mycelia Zinc Polysaccharides from Thelephora ganbajun TG-01. Oxidative medicine and cellular longevity. 2020:2308017.
Abstract
This study demonstrates that Thelephora ganbajun had a strong ability to absorb zinc, and zinc can be compartmentally stored in the small vesicles and mainly accumulated in the form of zinc-enriched polysaccharides (zinc content was 25.0 ± 1.27 mg/g). Mycelia zinc polysaccharides (MZPS) and its fractions were isolated. The main fraction (MZPS-2) with the highest antioxidant activity in vitro was composed of mannose : galacturonic acid : glucose : galactose in a molar ratio of 61.19 : 1 : 39.67 : 48.67, with a weight-averaged molecular weight of 5.118 × 105 Da. MZPS-2 had both α-pyranose and β-pyranose configuration and had a triple helical conformation. By establishing zebrafish models, we found that MZPS-2 can significantly scavenge free radicals, reduce the generation of reactive oxygen species caused by inflammation, and inhibit the recruitment of neutrophils toward the injury site. Therefore, MZPS-2 exhibited antioxidant and anti-inflammatory effects and can be used as a zinc supplement with specific biological activities to alleviate zinc deficiency complications, such as chronic oxidative stress or inflammation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping