FIGURE SUMMARY
Title

Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish

Authors
García-Poyatos, C., Cogliati, S., Calvo, E., Hernansanz-Agustín, P., Lagarrigue, S., Magni, R., Botos, M., Langa, X., Amati, F., Vázquez, J., Mercader, N., Enríquez, J.A.
Source
Full text @ EMBO Rep.
1

Characterization of <styled-content toggle='no' style='fixed-case'>OXPHOS</styled-content> super‐assembly in zebrafish

Split channels of BNGE of mouse (M) C57BL/6J (111), CD1(113), and zebrafish (ZF) muscle mitochondria shown in Fig 1A and B.

Immunodetection of the indicated proteins after 2D BNGE/DDM electrophoresis of whole‐body zebrafish mitochondria (representative of n = 3). Merged (D) and (E) split channels.
3

Diet‐induced recovery of <italic>scaf1</italic><sup>−/−</sup> phenotypes in males and diet effect in <styled-content toggle='no' style='fixed-case'>SCAF</styled-content>1‐deficient mice

Representative images of scaf1−/− and scaf1+/+ males fed with the indicated diets.

Size of males after the indicated diet. (B) Changes in length and (C) weight over time (Δ1 +/+ n = 10, Δ1 −/− n = 10, Δ2 +/+ n = 10, Δ2 −/− n = 7–8).

Effect of SCAF1 loss of function on weight gain in mice after starvation. (D) Scheme of the food restriction experiment in mice. (E) Impact of food restriction in C57BL/6JOlaHsd mice with the functional version of SCAF1 113/113, with the spontaneous mutation in SCAF1 111/111 (natural C57BL/6JOlaHsd mice harbor a non‐functional version of SCAF1) and in C57BL/6JOlaHsd mice without SCAF1 (SCAF1 KO, −/−). Males 111/111 n = 3; males KO n = 6; males 113/113 n = 2; females 111/111 n = 5; females KO n = 8; females 113/113 n = 5.

Data information: (B, C) Two‐way ANOVA. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. (E) Data are represented as mean ± SEM.
4

Transgenic expression of <italic>scaf1</italic> recovers <styled-content toggle='no' style='fixed-case'>CIII</styled-content> and <styled-content toggle='no' style='fixed-case'>CIV</styled-content> super‐assembly and fish size of <italic>scaf1</italic><sup>−/−</sup> fish

Scheme of the transgenic construct Tg(ubi:scaf1). ubi, ubiquitin promoter. Βg‐intron, beta‐globin intron, pA, poly A, crys, crystalline promoter (used as selection marker).

Immunodetection of the indicated proteins of BNGE from whole fish mitochondria scaf1Δ1/Δ1 (−/−) expressing the transgenic construct Tg(ubi:scaf1) in heterozygosis, their scaf1Δ1/Δ1 siblings with no transgenic expression, and scaf1+/+ zebrafish (representative BNGE of n = 5). Asterisks mark bands’ absence in scaf1Δ1/Δ1 (−/−) recovered by the transgenic expression of scaf1.

Representative images from scaf1+/+, scaf1Δ1/Δ1 (scaf1−/−), and scaf1−/−, Tg/− (C) female and (D) male zebrafish (3 mpf).

Size of scaf1+/+, scaf1Δ1/Δ1 (scaf1−/−), and scaf1−/−, Tg/− fish (E) length of females (scaf1+/+n = 23, scaf1−/−n = 39 and scaf1−/−, Tg/−n = 25); and (F) males (scaf1+/+n = 15, scaf1−/−n = 31 and scaf1−/−, Tg/−n = 44).

Data information: One‐way ANOVA. ns P > 0.05, **P < 0.01, ****P < 0.0001. Data are represented as mean ± SD.
6

Lack of recovery of Scaf1<sup>−/−</sup> phenotypes after high‐fat diet

Representative images of scaf1−/− and scaf1+/+ females (A) and males (B) fed with the indicated diets.

Length of females (C) and males (D) after the indicated diets (Δ1 +/+ n = 5, Δ1 −/− n = 5, Δ2 +/+ n = 5, Δ2 −/− n = 5).

Weight of females (E) and males (F) after the indicated diets (Δ1 +/+ n = 5, Δ1 −/− n = 5, Δ2 +/+ n = 5, Δ2 −/− n = 5).

Number of eggs per clutch (control diet: Δ1 +/+ n = 4, Δ1 −/− n = 3, Δ2 +/+ n = 3, Δ2 −/− n = 2, high fat diet: Δ1 +/+ n = 5, Δ1 −/−, n = 5 Δ2 +/+ n = 1, Δ2 −/− n = 2).

Quantification of mature ovary follicles per ovary section in hematoxylin–eosin (H&E) histological sections (average of three sections/biological sample; Δ1 n = 3, Δ2 n = 3).

Adipose tissue quantification in H&E sections (Δ1 n = 3, Δ2 n = 3) in females (I, K) and males (J, L): adipose tissue area (I, J) and adipocyte size (K, L).

 Data information: (C–F) Two‐way ANOVA. (G–L) Unpaired t‐test. Data are represented as mean ± SD. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
Fig. 5

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Rep.
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