FIGURE

6

ID

ZDB-FIG-200709-35

Publication

García-Poyatos et al., 2020 - Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish

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Lack of recovery of Scaf1<sup>−/−</sup> phenotypes after high‐fat diet

A, B

Representative images of scaf1^−/− and scaf1^+/+ females (A) and males (B) fed with the indicated diets.

C, D

Length of females (C) and males (D) after the indicated diets (Δ1 +/+ n = 5, Δ1 −/− n = 5, Δ2 +/+ n = 5, Δ2 −/− n = 5).

E, F

Weight of females (E) and males (F) after the indicated diets (Δ1 +/+ n = 5, Δ1 −/− n = 5, Δ2 +/+ n = 5, Δ2 −/− n = 5).

Number of eggs per clutch (control diet: Δ1 +/+ n = 4, Δ1 −/− n = 3, Δ2 +/+ n = 3, Δ2 −/− n = 2, high fat diet: Δ1 +/+ n = 5, Δ1 −/−, n = 5 Δ2 +/+ n = 1, Δ2 −/− n = 2).

Quantification of mature ovary follicles per ovary section in hematoxylin–eosin (H&E) histological sections (average of three sections/biological sample; Δ1 n = 3, Δ2 n = 3).

I–L

Adipose tissue quantification in H&E sections (Δ1 n = 3, Δ2 n = 3) in females (I, K) and males (J, L): adipose tissue area (I, J) and adipocyte size (K, L).

Data information: (C–F) Two‐way ANOVA. (G–L) Unpaired t‐test. Data are represented as mean ± SD. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cox7a2l^brn1/brn1 cox7a2l^brn2/brn2
Condition:	high fat
Observed In:	female organism female organism adipose tissue female organism fat cell male organism male organism adipose tissue male organism fat cell oocyte ovarian follicle stage IV
Stage:	Adult

Phenotype Detail

Acknowledgments

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