ZFIN Image: García-Poyatos et al., 2020, 4

Image

Figure Caption

4 Transgenic expression of <italic>scaf1</italic> recovers <styled-content toggle='no' style='fixed-case'>CIII</styled-content> and <styled-content toggle='no' style='fixed-case'>CIV</styled-content> super‐assembly and fish size of <italic>scaf1</italic><sup>−/−</sup> fish

A

Scheme of the transgenic construct Tg(ubi:scaf1). ubi, ubiquitin promoter. Βg‐intron, beta‐globin intron, pA, poly A, crys, crystalline promoter (used as selection marker).

B

Immunodetection of the indicated proteins of BNGE from whole fish mitochondria scaf1^Δ1/Δ1 (−/−) expressing the transgenic construct Tg(ubi:scaf1) in heterozygosis, their scaf1^Δ1/Δ1 siblings with no transgenic expression, and scaf1^+/+ zebrafish (representative BNGE of n = 5). Asterisks mark bands’ absence in scaf1^Δ1/Δ1 (−/−) recovered by the transgenic expression of scaf1.

C, D

Representative images from scaf1^+/+, scaf1^Δ1/Δ1 (scaf1^−/−), and scaf1^−/−, Tg/− (C) female and (D) male zebrafish (3 mpf).

E, F

Size of scaf1^+/+, scaf1^Δ1/Δ1 (scaf1^−/−), and scaf1^{−/−, Tg/−} fish (E) length of females (scaf1^+/+n = 23, scaf1^−/−n = 39 and scaf1^{−/−, Tg/−}n = 25); and (F) males (scaf1^+/+n = 15, scaf1^−/−n = 31 and scaf1^{−/−, Tg/−}n = 44).

Data information: One‐way ANOVA. ns P > 0.05, **P < 0.01, ****P < 0.0001. Data are represented as mean ± SD.

Figure Data

Phenotype details

Acknowledgments

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