FIGURE SUMMARY
Title

Characterization of paralogous uncx transcription factor encoding genes in zebrafish

Authors
Nittoli, V., Fortunato, A.E., Fasano, G., Coppola, U., Gentile, A., Maiella, S., Langellotto, F., Porreca, I., De Paolo, R., Marino, R., Fiengo, M., Donizetti, A., Aniello, F., Kondo, T., Ristoratore, F., Canzoniero, L.M.T., Duboule, D., Wilson, S.W., Sordino, P.
Source
Full text @ Gene X
Fig. 3

Expression of uncx4.1 gene during somitogenesis. Whole-mount in situ hybridization of uncx4.1 at (A) 11 hours post fertilization (hpf), (B) 11.5 hpf, (C) 13 hpf, (D) 14.5 hpf, (E) 19 hpf, (F) 24 hpf, and (G–L) 34 hpf. (A, C, E–H, J, K) Lateral view, (B, D) dorsal view, and (I) transversal section. (A–H, J–L) Anterior to left, and (I) toward viewer. (A) Expression in early somites (So) at 11 hpf. HB = hindbrain. (B–D) Expression in somites from 11.5 to 14.5 hpf. Dotted lines show boundary between presomitic mesoderm (PSM) and last formed somite. (E) Expression is lost in the myoseptum and is restricted posteriorly in developing somites at 10 hpf. (F) Expression is restricted to dorsal and ventral margins of anterior somites at 24 hpf, and (G) disappears dorsally at 34 hpf. CS = Corpuscle of Stannius, So = somites. (H) Arrowheads indicate in situ hybridization staining in ventro-latero-posterior cells (VLP) at 34 hpf. (I) Arrowheads indicate VLP cells in phalloidin-stained somites at 34 hpf. (J) Arrowhead indicates expression in VLP cells in transversal section at 16th somite level at 34 hpf. (K, L) Arrows indicate expression in sclerotome cells in (K) lateral and (L) dorsal view at 34 hpf.
Fig. 4

Non-somitic expression of uncx4.1. Whole-mount in situ hybridization of uncx4.1 at (A) tailbud stage, (B–D) 18 hours post fertilization (hpf), (E, F) 24 hpf, (G–L) 34 hpf, and (M) 48 hpf. (A, C, F, H, J) Dorsal view, (B, E, G, H, K–M) lateral view, and (D, I) frontal view. (A, C) Anterior to top, (B, E–H, J–M) to left, and (D, I) toward viewer. (A) Early expression in rhombomeres 3–5 (r3, r5) as shown by double in situ labeling with egr2b riboprobe. Expression in telencephalon (Te), olfactory placodes (OP), hypothalamus (Hy), and prospective hindbrain (HB) at (B–D) 18 hpf and (E, F) 24 hpf. Di = diencephalon, HB = hindbrain, Hy = hypothalamus, OP = olfactory placode, Te = telencephalon. (G, H) Expression extends to thalamus at 34 hpf. Ce = cerebellum, Ey = eye, Th = thalamus. (I) Transversal section showing expression in neuronal progenitor cells in the hindbrain at 34 hpf. (J) Arrowheads indicate expression in the hindbrain near exit roots of branchial motor neurons as shown by double in situ labeling with acetylated α-tubulin antibody at 34 hpf. Expression (K) in pharyngeal arches and (L) Corpuscle of Stannius at 34 hpf in lateral view. CS = Corpuscle of Stannius, PA = pharyngeal arches, YE = yolk extension. (M) Expression in central nervous system and (inset) Corpuscle of Stannius at 48 hpf. OT = optic tectum, Yo = yolk.
Fig. 5

Expression of uncx during embryogenesis. Whole-mount in situ hybridization of uncx at (A) 12.5 hours post fertilization (hpf), (B–E) 18 hpf, (F) 22 hpf, (G, H) 24 hpf, (I) 34 hpf, and (J, K) 48 hpf. (A, C–E, K) Dorsal view, and (B, F–J) lateral view. Anterior to left. (A) Expression in prospective forebrain (FB) and hindbrain (HB) at 12.5 hpf. FB = forebrain, PSM = presomitic mesoderm, So = somites. (B–E) Expression of uncx at 18 hpf. HB = hindbrain, Hy = hypothalamus, No = notochord, OP = olfactory placode, SC = spinal cord, So = somites. (F) Expression at 22 hpf. Th = thalamus. (G, H) Expression at 24 hpf. Ce = cerebellum, Te = telencephalon. (I) Expression at 34 hpf. (J, K) Expression at 48 hpf.
Fig. 6

Expression of uncx4.1 during somite patterning and formation. Whole-mount in situ hybridization of uncx4.1 at (A) 12.5 hours post fertilization (hpf), (B) 19 hpf, (C) 12 hpf, (D, E) 15.5 hpf, (F) 13 hpf, (G, H) 18.5 hpf, (I) 14 hpf, and (L) 22 hpf. (A, B, F, G, L) Lateral view, and (C–F, H, I) dorsal view. (A–D, F–L) Anterior to left, and (E) to top. (A, B) Expression in presomitic mesoderm as shown by double in situ labeling with her1 and mespaa riboprobes. PSM = presomitic mesoderm. Dotted lines show boundary between presomitic mesoderm (PSM) and last formed somite. (C–E) Expression is absent in adaxial cells and colocalizes with myoD1 expression in muscle progenitor cells as shown by double in situ labeling with myoD1 riboprobe at (C) 12 hpf, and (D, E) 15.5 hpf. (F–H) Expression during muscle fibre differentiation as shown by double in situ labeling with the MF20 antibody at (F) 13 hpf, and (G, H) 18.5 hpf. (I, L) Expression throughout somitic mesoderm in fused somite mutant embryos at (I) 14 hpf, and (L) 22 hpf.
Fig. 7

Regulation of uncx gene expression by Hedgehog (Hh) signaling pathway. Whole-mount in situ hybridization of (A–F, H–L, O, P) uncx4.1 and (G, M, N) uncx at (A, B, E, G) 19 hours post fertilization (hpf), (C, D) 24 hpf, (F) 30 hpf, (H, O, P) 34 hpf, (I, J) 14.5 hpf, and (K–N) 22 hpf. (A, C, P) Dorsal view, (B, E–O) lateral view, and (D) frontal view. (A–C, E–P) Anterior to left, and (D) toward viewer. (A, B) Spatial relationship between uncx4.1 expression and Hh signal-releasing notochord as shown by double in situ labeling with shha riboprobe at 19 hpf in (A) dorsal and (B) lateral view. No = notochord. (C, D) Loss of uncx4.1 expression in shha mRNA-injected side at 24 hpf in (C) lateral and (D) frontal view. (E–G) Expression of (E, F) uncx4.1 and (G) uncx in sonic-you (syu) mutant embryos at (E, G) 19 hpf and (F) 30 hpf (controls in Fig. 3E, 5B, and 3H, respectively). (F) Arrowheads indicate uncx4.1-expressing cells. SC = spinal cord, So = somites. (H) Expression of uncx4.1 in slow-muscle-omitted (smu) mutant embryos at 34 hpf. (I, J) Expression of uncx4.1 in (I) sibling (sib) and (J) you-too (yot) mutant embryos at 14.5 hpf. Arrowheads indicate anterior margin of expression in somites. (K–N) Expression of (K, L) uncx4.1 and (M, N) uncx in embryos treated with (K, M) EtOH and (L, N) cyclopamine A at 22 hpf in lateral view. (O, P) Expression of uncx4.1 in floating head (flh) mutant embryos at 34 hpf (controls in Fig. 3H and I, respectively).
Fig. 8

Regulation of uncx gene expression in relation to Fgf, Nodal and Notch/Delta signaling pathways. Whole-mount in situ hybridization of (A, E, H, I) uncx4.1 and (B–D, E, G, J) uncx at (A) 34 hpf, (B–D) 19 h post fertilization (hpf), (E, F, H) 24 hpf, (G) 18 hpf, and (J) 19 hpf. Lateral view, anterior to left. Expression of (A) uncx4.1 and (B) uncx in acerebellar (ace) mutant embryo at (A) 34 hpf and (B) 19 hpf. (C, D) Expression of uncx in embryos treated with (C) DMSO and (D) SU5402 at 19 hpf. (E, F) Expression of (E) uncx4.1 and (F) uncx in cyclops (cyc) mutant embryo at 24 hpf (controls in Fig. 3F and 5H, respectively). (G) Expression of uncx in embryo treated with SB431542 at 18 hpf (control in Fig. 5B). (H–J) Expression of (H, I) uncx4.1 and (J) uncx in embryos treated with (H) DMSO and (I, J) DAPT at (H, I) 24 hpf and (J) 19 hpf. (J) Control in (C).
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EXPRESSION / LABELING:
Gene:
Fish:
Stage Range: Prim-15 to Long-pec
Acknowledgments
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