Regulation of uncx gene expression in relation to Fgf, Nodal and Notch/Delta signaling pathways. Whole-mount in situ hybridization of (A, E, H, I) uncx4.1 and (B–D, E, G, J) uncx at (A) 34 hpf, (B–D) 19 h post fertilization (hpf), (E, F, H) 24 hpf, (G) 18 hpf, and (J) 19 hpf. Lateral view, anterior to left. Expression of (A) uncx4.1 and (B) uncx in acerebellar (ace) mutant embryo at (A) 34 hpf and (B) 19 hpf. (C, D) Expression of uncx in embryos treated with (C) DMSO and (D) SU5402 at 19 hpf. (E, F) Expression of (E) uncx4.1 and (F) uncx in cyclops (cyc) mutant embryo at 24 hpf (controls in Fig. 3F and 5H, respectively). (G) Expression of uncx in embryo treated with SB431542 at 18 hpf (control in Fig. 5B). (H–J) Expression of (H, I) uncx4.1 and (J) uncx in embryos treated with (H) DMSO and (I, J) DAPT at (H, I) 24 hpf and (J) 19 hpf. (J) Control in (C).
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