Influence of dexamethasone (Dex) concentration on zebrafish skull. Schematic diagram of TG(SP7:EGFP) zebrafish larvae (A,B). IOD values of green fluorescence of Dex-induced bone damage in TG zebrafish larvae (C). Images of green fluorescence in TG zebrafish larvae skull [in profile (D)]. The Dex concentrations used were 0, 0.25, 5.00, 10.00, 15.00, 20.00, and 25.00 μmol/l, and 0.1% DMSO was used as the control. n = 15, ***p < 0.01.

IC₅₀ and area of bone mineralization after Dex-induced bone damage in AB-strain zebrafish larvae. The 50% inhibitory concentration of dexamethasone (Dex) (A). Analysis of mineralization area in the skull and spine of Dex-treated AB-strain zebrafish larvae at 9 dpf (B). Alizarin red S staining of the skull in Dex-treated AB-strain zebrafish larvae at 9 dpf (C). The Dex concentrations used were 0.25, 1.00, 5.00, 10.00, 15.00, 20.00, and 25.00 μmol/l. The zebrafish larvae were exposed to Dex from 3 to 9 dpf, and 0.1% DMSO served as the control. n = 15, *p < 0.05, ***p < 0.01.

Effect of Res on Dex-induced bone damage in zebrafish. IOD values of green fluorescence of Res after Dex-induced bone damage in TG zebrafish larvae (A). Images of green fluorescence in TG zebrafish larvae skull [in profile (B)]. The Res concentrations used were 25.00, 50.00, 75.00, 100.00, 150.00, 200.00, and 250.00 μmol/l, and 0.1% DMSO served as the control. The area of bone mineralization after Res treatment of Dex-induced bone damage in AB-strain zebrafish larvae at 9 dpf [(C) 15.00 μmol/l Dex vs. 15.00 μmol/l Dex plus 150.00 μmol/l Res]. Alizarin red S staining of the skull after Res treatment of Dex-induced bone damage in AB-strain zebrafish larvae at 9 dpf (D). n = 15, *p < 0.05, ***p < 0.01.