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Fig. 2
IC50 and area of bone mineralization after Dex-induced bone damage in AB-strain zebrafish larvae. The 50% inhibitory concentration of dexamethasone (Dex) (A). Analysis of mineralization area in the skull and spine of Dex-treated AB-strain zebrafish larvae at 9 dpf (B). Alizarin red S staining of the skull in Dex-treated AB-strain zebrafish larvae at 9 dpf (C). The Dex concentrations used were 0.25, 1.00, 5.00, 10.00, 15.00, 20.00, and 25.00 μmol/l. The zebrafish larvae were exposed to Dex from 3 to 9 dpf, and 0.1% DMSO served as the control. n = 15, *p < 0.05, ***p < 0.01.
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