Developmental parameters and mRNA expression of developmental genes in fushi tarazu factor 1b (ff1b) knockout zebrafish by CRISPR/Cas9 technology. (A-D) Developmental parameters of F₃ zebrafish embryos; (A) body lengths; (B) intrauterine growth retardation (IUGR) rates; (C) the whole body of F₃ wild-type (WT) embryos at 6 days post-fertilization (dpf); (D) the whole body of F₃ mutant-type (MT) embryos at 6 dpf; (E) the yolk and swim bladder of F₃ WT embryos at 6 dpf; (F) the yolk and swim bladder of F₃ MT embryos at 6 dpf. White arrow: swim bladder; black arrow: yolk. (G) Segment number; (H) general morphology score (GMS). Mean ± SD, ^*P<0.05, two-sided t-test, n = 3 F₂ zebrafish. Real-time reverse-transcription PCR (RT-PCR) was used to detect the mRNA expression of developmental genes in F₃ embryos. (I) goosecoid (gsc) expression in F₃ embryos at 12 hours post-fertilization (hpf); (J) krox20 expression in F₃ embryos at 12 hpf. Mean ± SD, ^*P<0.05, two-sided t-test, n = 3 embryos.

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