Localisation of bpifcl mRNA expression in zebrafish brain. (A) Schematic sagittal drawing of the zebrafish brain. (B–F) Lines in A indicate levels of coronal sections. Schematic coronal brain drawing of zebrafish showing the distribution of bpifcl (red dots) mRNA-containing cells in the brain. (Ga–Gh) Photomicrographs of bpifcl-expressing cells in 6 months old female zebrafish brain. (Ha–Hh) Photomicrographs of bpifcl-expressing cells in 6 months old male zebrafish brain. (Ia–Ih) Photomicrographs of bpifcl-expressing cells in 60 days post-fertilisation (dpf) fish brain. For abbreviations, see Supplementary Abbreviation. Scale bar: 50 μm.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Photomicrographs of double-label fluorescence ISH of bpifcl and kiss2 mRNA, and effect of knocked-down bpifcl mRNA expression. Anti-sense riboprobe-labelled bpifcl (A) and kiss2 (B) mRNA was co-expressed in the dorsal zone of the periventricular hypothalamus (Hd) (C). Scale bars: 20 μm. Localisation of kiss2 mRNA in the Hd of GFP siRNA injected female (D) and bpifcl siRNA injected female (E). Scale bars: 20 μm. bpifcl (F) and kiss2 (G) mRNA expression in bpifcl siRNA-injected female zebrafish, and bpifcl (H) and kiss2 (I) mRNA expression in bpifcl siRNA-injected males. Expression levels of GFP siRNA injected fish were defined as 1.0. All data are presented as the mean ± standard error of the mean and analysed by a Student’s t-test. *p < 0.05.