ZFIN Image: Moriya et al., 2017, Fig. 4

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Figure Caption

Fig. 4

Photomicrographs of double-label fluorescence ISH of bpifcl and kiss2 mRNA, and effect of knocked-down bpifcl mRNA expression. Anti-sense riboprobe-labelled bpifcl (A) and kiss2 (B) mRNA was co-expressed in the dorsal zone of the periventricular hypothalamus (Hd) (C). Scale bars: 20 μm. Localisation of kiss2 mRNA in the Hd of GFP siRNA injected female (D) and bpifcl siRNA injected female (E). Scale bars: 20 μm. bpifcl (F) and kiss2 (G) mRNA expression in bpifcl siRNA-injected female zebrafish, and bpifcl (H) and kiss2 (I) mRNA expression in bpifcl siRNA-injected males. Expression levels of GFP siRNA injected fish were defined as 1.0. All data are presented as the mean ± standard error of the mean and analysed by a Student’s t-test. *p < 0.05.

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Expression / Labeling details

Acknowledgments

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